Abstract

The wastewater in Bagru and Jharna area (Rajasthan) is rich in pathogenic and non-pathogenic keratinophilic fungi including dermatophytes. Most of the fungal isolates from the waste water have already been reported as dermatophytes causing infections of skin, scalps or hair. This study deals with the isolation and characterization of keratinophilic fungi from different water samples of Bagru and Jharna area (Rajasthan). Eighteen different fungi were isolated out of twenty water samples using a spread plate method. These fungal isolates were Epidermatophyton floccosum, Microsporum ferrugineum,Microsporum gypseum, Microsporum cookei, Microsporum canis, Trichophyton rubrum,Trichophyton mentagrophytes, Trichophyton concentricum, Trichophyton verrucosum,Trichophyton violaceum, other fungi were Aspergillus niger, Aspergillus fumigatus,Aspergillus flavus, Aspergillus terreus, Rhizopus oryzae, Penicillium sp., Fusarium oxysporum and Chrysosporium tropicum. Our result concluded that these isolated fungi are pathogenic for animals and human beings. Keratinophilic fungi play an important role in degradation of keratin and they are known to cause superficial cutaneous infections.   Key words: Keratinophilic fungi, pathogenic, non-pathogenic, spread plate method, superficial cutaneous infections, dermatophytes.

Highlights

  • Polluted water is a dynamic medium in which a large number of keratinophilic and non-keratinophilic fungus live in close association

  • Our present study reports the occurrence of keratinophilic fungi in the water samples of Bagru and Jharna area (Rajasthan) which causes superficial mycosis of human beings and animals

  • The data reveals that out of twenty water samples collected, eighteen species of keratinophilic and non- keratinophilic fungi were isolated from different water samples with different keratin substrates such as hair nails and feathers

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Summary

Introduction

Polluted water is a dynamic medium in which a large number of keratinophilic and non-keratinophilic fungus live in close association. The first discovery of keratinophilic fungi from waste water was conducted using membrane bioreactors (MBR) (Awad and Kraume, 2011). These fungi are natural colonizers on keratinic substances. Because of tight packing of hair polypeptide chain in α- helix structures and their linkage by disulphide bonds, they are poorly biodegradable (Kunert et al, 2000). This tough, fibrous and insoluble material provided an outer coat that served to prevent the loss of body fluids. According to sulphur contents keratins are grouped into hard keratin that is, feather, hair, hoof and nails or soft keratins that is, skin and Sharma et al 6749

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