Abstract
Nanotechnology is rapidly growing with nanoparticles produced and utilized in a wide range of pharmaceutical and commercial products throughout the world. In this study, fig (Ficus carica) leaf extracts were used for ecofriendly extracellular synthesis of stable silver nanoparticles (AgNPs) by treating an aqueous silver nitrate (1 mM) solution and using the plant F. carica leaf extracts as reducing agents. The bioreduced silver nanoparticles were characterized by ultra violet visible (UV-Vis) spectrophotometer, Fourier transform infra-red (FTIR) spectroscopy and transmission electron microscopy (TEM). The average particle size ranged from 5 to 40 nm. The particle size could be controlled by changing the reaction temperature, leaf broth concentration and AgNO3 concentration. Further, these biologically synthesized nanoparticles concentration of 50 µl were found to be highly effective and exhibited maximum microbial activity with mean zone of inhibition 20.33±1.00 mm and 18.00±1.00 against pseudomonas aeruginosa and Aspergillus fumigatus isolated from human corneal ulcer patients. This environmentally friendly green synthesis is an eco-friendly approach to conventional chemical synthesis and can potentially be used in various areas such as food, cosmetics, and medical applications and hope the recent technology can provide next generation of anti-microbials. Key words: Ficus carica, silver nanoparticles, characterization, antimicrobial activity.
Highlights
AgNPs) synth hesis a and c charactterization of fig Fic cus carrica leaff extrac ct and its an ntimicro obial effect e agains a st clinic cal iso olates ffrom corn neal ulc cer Yousef H
F. carica leaves were collected from Riyadh market, and the species was identified with the authenticated specimen from the Department of Agriculture, Qassim University, Kingdom of Saudi
Synthesized silver nanoparticles was confirmed by sampling the aqueous component of different time intervals and the absorption maxima was scanned by ultra vio olet visible (UV-Vis) spectrophotometer at the wavelength of 300 to 800 nm on Perkin-Elmer Lambda 25 spectrophotometer
Summary
AgNPs) synth hesis a and c charactterization of fig Fic cus carrica (fig) leaff extrac ct and its an ntimicro obial effect e agains a st clinic cal iso olates ffrom corn neal ulc cer Yousef H. Departmen nt of Optomettry, College of o Applied Med dical Science es, Qassim Un niversity, King gdom of Saud di Arabia. Dep partment of Pa athology, Colllege of Veterinary Medicin e, Suez Cana al University, Ismailia, Egyypt
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