Abstract
Along with other emerging health consequences, diabetes mellitus is one of the critical threats spreading all over the world with continuity. The investigation of glucose level in diabetic patients requires the key enzyme β-D-glucose:oxygen-1-oxidoreductase/glucose oxidase, where its optimized production by mutant derived strain could affect the economic burden and accuracy of the test. Wild typeAspergillus niger was subjected to ultraviolet radiation for enhanced production of glucose oxidase. It was found that UV-180-C is a potential mutant derived strain, screened by using 2-deoxy-D-glucose. Optimum production of glucose oxidase from A. niger UV-180-C was carried out by using CSL (2%), fermentation period (36 h), pH (5.5 and 4.5 for wild and mutant respectively), temperature (30°C), MgSO4.7H2O (0.0%), CaCO3 (0.1%), KH2PO4 (0.8 and 1.0% for wild and mutant strains), Urea (0.3%). Crude enzyme was subjected to ammonium sulfate precipitation and resulted into 145.8 UmL-1 activity. Glucose oxidase from mutant derived A. niger exhibited optimum pH at 6, temperature 20°C, Km 10mM and Vmax142 UmL-1. The pyridoxal phosphate caused significant inhibition to the enzyme which indicates the presence of lysyl residues near or at the active site of the enzyme from both wild and mutant derived strains. Key words: Enhanced production, UV radiations, β-D-glucose:oxygen-1-oxidoreductase, A. niger
Highlights
Diabetes mellitus is a general metabolic problem resulting in serious consequences which cause death
Wild type Aspergillus niger was subjected to ultraviolet radiation for enhanced production of glucose oxidase
The production optimization is an area of great interest for the maximal production of glucose oxidase using the mutant strains of Aspergillus niger
Summary
Diabetes mellitus is a general metabolic problem resulting in serious consequences which cause death. The diagnostic methods for diabetes have been compared to find the accurate method for glucose. The enzymatic method using glucose oxidase enzyme was most advantageous (Giampietro et al, 1982). The total cost for the diabetes care has been investigated for the Pakistan in out patient clinics of Pakistan in 2007, that reported Rs. 515.8 are required per visit (Liaqat et al, 2007). The cost reduction at the diagnostics level could relief the economic pressure on the patients. Research has been focused on the enhanced production of enzymes by using mutant derived strains (Khattab and Bazaraa, 2005; Haq et al, 2001). The production optimization is an area of great interest for the maximal production of glucose oxidase using the mutant strains of Aspergillus niger
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