Abstract
Lallemantia royleana (Benth.) is an important folk medicine in Pakistan. This natural herb is used as folk remedy for number of ailments. This study was conducted to assess the antibacterial activity of four different organic extracts of L. royleana seeds against four bacterial strains (Escherichia coli, Enterobacter cloaceae, Pseudomonas aeruginosa and Staphylococcus aureus) for the first time by disc diffusion method. Except aqueous extracts, all organic extracts of L. royleana seeds displayed significant anti-bacterial activity against all the tested bacteria. The chloroform extract exhibited highest anti-bacterial activity for all bacterial strains. Results shows that L. royleana seeds possess significant antibacterial potential against S. aureus, E. coli and E. cloaceae, therefore, it can be a good remedy for skin disease and gastro-intestinal problems caused by human pathogenic bacterial strains. Further screening for phytochemicals should be carried out in search of novel therapeutic compounds.
Highlights
Molec cular charact c terizatiion and in viitro eva aluatio on of endop phytic bacterria aga ainst m major p pathogens off rice
Among the endophytic e bacterial b iso olates tested against the e major path hogens of rice viz., Pyricularia grrisea, Rhizoc ctonia solan ni and Xanth homonas ory ryzaepv. oryzzae in vitro, Bacillus subtilis varr. amylolique efaciens
Bacterial cells were harvested by centrifugation at 12,000 g for 15 min and bacterial cells were resuspended in phosphate buffer (PB) (0.01 M, pH 7.0)
Summary
De epartment of Plant Patholo ogy, Center fo or Plant Prote ection Studiess, Tamil Nadu u Agricultural University, Co oimbatore-3, India. Bacillus su to screen ag gainst majorr pathogens of o rice. All th he isolates w were characte erized on the e basis of bio ochemical and phenottypic analysis. E, following these tests, iit can be con ncluded that 31 isolates exhibited differences and they were w subjectted to partia al 16S-rDNA. Among the endophytic e bacterial b iso olates tested against the e major path hogens of rice viz., Pyricularia. Pla ants are consttantly involve ed in interactio ons with a wide range of bactteria These plant-assoc ciated bacte eria colo onize the rhiz zosphere (rhiz zobacteria), th he phyllosphe ere hytes) and iinside the pllant tissues (endophytes)).
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