Abstract
The emergent Salmonella typhimurium definitive phage type (DT) 104 is of particular global concern due to its frequent isolation and multiple antibiotic resistances. There is thus a need to know the kind of proteins expressed by S. typhimurium DT104 so as to provide a basis for developing an intervention. This study examined the protein profiles of a few isolates of S. typhimurium DT104 and a non-DT104 strain S. typhimurium L1388 (ST). Crude SDS-soluble whole cell and outer membrane protein (OMP) extracts revealed similar protein profiles for both phage types. A single major protein band (28.4 kDa) was observed in periplasmic fractions from both phage types. However, proteins released into growth medium was variable; one of the DT104 isolates had common proteins with the non-DT104 strain ST. Similar SDS-soluble whole cell protein profiles were observed for both phage types grown in a low-iron Medium A at 37oC; but a 38.5 kDa protein (observed in TSB-grown cells) was observed only in the temperature-tolerant DT104 isolate. The protein contents of cell-free ultracentrifuge supernatants of sonically disrupted cells of each of the DT104 isolates were significantly (P African Journal of Biotechnology Vol. 4 (7), pp. 727-737, 2005
Highlights
In human, swine, avian and bovine salmonellosis, Salmonella typhimurium is the most frequently isolated serotype out of the over 2,400 serotypes in the genus Salmonella (Schwartz, 1999; Popoff, 1997)
We examined the profiles of five human isolates of the multi-resistant strain S. typhimurium DT104 and a non-multi-resistant strain S. typhimurium L1388, with the hope of finding differences or otherwise in the protein profiles
SDS-soluble whole cell lysates of all the DT104s and S. typhimurium L1388 (ST) indicated similar banding patterns. 2DE maps of Whole cell (WC) proteins of selected isolates grown in the same media were similar (Figures 1B, C and D)
Summary
Swine, avian and bovine salmonellosis, Salmonella typhimurium is the most frequently isolated serotype out of the over 2,400 serotypes in the genus Salmonella (Schwartz, 1999; Popoff, 1997). The emergence and spread of the definitive phage type strain (DT104) is an important human and veterinary concern globally, because of the strain’s multiple-drug resistances and extensive host range (Baggessen et al, 2000; Sameshima et al, 2000; Poppe et al, 1998; Threlfall et al, 1996; Akkina et al, 1999; Besser et al, 1997) An infection with this strain, in humans, is associated with common symptoms such as diarrhea, fever, abdominal pain, vomiting, and blood in the stool (Poppe et al, 1998).
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