Abstract
An efficient protocol was described for the rapid in vitro multiplication of an endangered medicinal plant, Caralluma bhupenderiana Sarkaria, via enhanced axillary bud proliferation from nodal explants collected from young shoots of six-months-old plant. The physiological effects of growth regulators [6-Benzyladenine (BA), kinetin (Kn), 2-Isopentyl adenine (2iP), zeatin (Zn), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA)], different strengths of Murashige and Skoog (MS) medium and various pH levels on in vitro morphogenesis were investigated. The highest number (8.40 + 0.50) of shoots and the maximum average shoot length (3.2 ± 0.31 cm) were recorded on MS medium supplemented with BA (8.87 µM) at pH 5.8. Rooting was best achieved on half-strength MS medium augmented with NAA (2.69 µM). The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing peat mass and garden manure in 1:1 ratio and grown in a greenhouse with 80% survival rate. The regenerated plants did not show any immediate detectable phenotypic variation. Key words: Apocynaceae, asclepiadoideae, conservation, micro-propagation, endangered, Caralluma bhupenderiana.
Highlights
Caralluma R.Br. has been usually accepted to include about 120 taxa, with a wide African, Asian, South African and Southeast European distribution (Mabberley, 1993)
An efficient protocol was described for the rapid in vitro multiplication of an endangered medicinal plant, Caralluma bhupenderiana Sarkaria, via enhanced axillary bud proliferation from nodal explants collected from young shoots of six-months-old plant
It belongs to subtribe Stapeliinae, which has its centre of origin in East Africa (Meve and Liede, 2004)
Summary
Caralluma R.Br. (sensu lato.) has been usually accepted to include about 120 taxa, with a wide African, Asian, South African and Southeast European distribution (Mabberley, 1993). (sensu lato.) has been usually accepted to include about 120 taxa, with a wide African, Asian, South African and Southeast European distribution (Mabberley, 1993). It belongs to subtribe Stapeliinae (tribe Ceropegiae, sub family Asclepiadoideae and family Apocynaceae), which has its centre of origin in East Africa (Meve and Liede, 2004). Found in dry regions of the world, has paramount medicinal importance and has significant anti-inflammatory and antitumor activity (Deepak et al, 1997; Ramesh et al, 1999; Zakaria et al, 2001). It was observed that the administration of C. sinaica in different doses to healthy animals can cause
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