Abstract
The Caatinga biome occupies some 12% of the Brazilian territory, which is present in at least nine states. The species that constitute its biodiversity have the potential to be used as natural resources, among them are approximately 700 species of the genusCroton. As an example of this potential, the Croton linearifolius specie is used by local communities as a natural insecticide. Associated with the economic potential of the Caatinga species, one must stress the risk of extinction or genetic erosion due to the growing deforestation of natural areas of this biome. These factors make it relevant in genetic studies in order to guide conservation strategies. Considering the lack of molecular studies involving C. linearifolius, we compared the efficiency of six protocols for genomic DNA extraction previously described in literature. The DNA extraction buffers [based on the use of Cetyl trimethylammonium bromide (CTAB),sodium dodecyl sulfate (SDS), mannitol and sorbitol] were different in their efficiency to obtaining the genomic DNA of C. linearifloius. In general, protocols using CTAB buffer were more efficient. The use of liquid nitrogen in the maceration process was also evaluated and its use was considered a no necessary factor in obtaining DNA in adequate quantity and quality for PCR platform procedures. Key words: DNA Isolation, molecular markers, native species of Caatinga.
Highlights
IntroductionThe Caatinga biome occupies about 12% of the Brazilian territory, including the states of Bahia, Sergipe, Alagoas, Pernambuco, Paraíba, Rio Grande do Norte, Ceará, Piauí and the north of Minas Gerais (Almeida-Cortez et al, 2007)
Faced with the environmental characteristics presented, the Caatinga vegetation is adapted to water deficiency conditions, and produces tree, shrub and moss layers characterized by water-retaining structures, and/or superficial roots (Almeida-Cortez et al, 2007)
The DNA extraction buffers assessed are established for different species (Ojeda, 2012; Souza, 2012; Mogg and Bond, 2003; Štorchová et al, 2000) they differ in their efficiency in obtaining C. linearifolius genomic DNA (p
Summary
The Caatinga biome occupies about 12% of the Brazilian territory, including the states of Bahia, Sergipe, Alagoas, Pernambuco, Paraíba, Rio Grande do Norte, Ceará, Piauí and the north of Minas Gerais (Almeida-Cortez et al, 2007). The Caatinga vegetation is considered xerophytic, with diverse aspect and floristics, average rainfall between 240 and 1500 mm, and temperature ranging between 24 and 29°C (Almeida-Cortez et al, 2007). Faced with the environmental characteristics presented, the Caatinga vegetation is adapted to water deficiency conditions, and produces tree, shrub and moss layers characterized by water-retaining structures, and/or superficial roots (Almeida-Cortez et al, 2007). With such typical environmental characteristics, the Caatinga harbors large quantities of endemic species.
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