Abstract
A system for induction of callus and plant regeneration via somatic embryogenesisfrom root explants of Hevea brasiliensis Muell. Arg. clone Reyan 87-6-62 was evaluated. The influence of plant growth regulators (PGRs) including 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (6-BA) and kinetin (KT) on callus induction of root explants from in vitro plantlets were studied. The results showed that the highest induction frequency of embryogenic callus emerged on Murashige and Skoog (MS) medium supplemented with 1 mg/l KT, 0.2 mg/l 6-BA without 2,4-D. Mean of 4 somatic embryos per embryogenic callus were obtained and approximately 11.8% of them developed into plantlets. The regenerated plantlets were successfully transplanted to sand bed. The plant regeneration system established in this study will facilitate mass propagation and may be applied to culture the roots of high-yielding rubber trees. Key words: Hevea brasiliensis Muell. Arg., root explants, callus, somatic embryogenesis, regeneration.
Highlights
The rubber tree (Hevea brasiliensis) which is the main source of natural rubber is a heterozygous perennial tree with a long reproductive maturity
Biotechno (PGRs) on callus induction from root explants (Figure 1a), sixteen induction media based on Murashige and Skoog (MS) medium containing different concentrations of 2,4-D, KT and 6-BA were designed with orthogonal design
Type I callus was obtained on MS2 - MS16 medium containing different concentrations of plant growth regulators (PGRs), while type II was obtained on MS2-MS14
Summary
The rubber tree (Hevea brasiliensis) which is the main source of natural rubber is a heterozygous perennial tree with a long reproductive maturity. This makes improvement via conventional breeding very difficult. The interaction between rootstock and scion of grafted rubber tree affects growth, yield, as well as the physiological and biochemical characteristics of rubber trees Micropropagation of the rootstocks of high-yielding rubber trees has great significance for uniform growth and largely increases rubber output. The roots of the rootstocks can be a good explant source
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