Abstract
In this present study, biosurfactant-producing microorganisms Pseudomonas aeruginosa PBSC1, was isolated from mangrove ecosystem in Pichavaram (Boat house), Tamil Nadu, India. The biosurfactant production was done using a minimal salt medium (MSM) with crude oil as the hydrocarbon. The microbial growths were investigated, and the best cultivation times for the biosurfactant production by P. aeruginosa PBSC1 was found to be 144 h. The biosurfactant was extracted and characterized. The critical micelle concentrations (CMCs) of extracted biosurfactant was found to be 78 mg l -1 . Stability studies were done at various pH, temperature and NaCl concentrations. The emulsification activity was stable at lower and higher pH and temperature respectively. NaCl concentration does not affect the emulsification activity of the biosurfactant. The emulsion formed by the biosurfactant against each hydrocarbon was stable for one month. Further characterization of biosurfactant using Fourier transform infrared spectroscopy (FTIR) revealed it as a rhamnolipid. Keywords: Mangrove ecosystems, Pseudomonas aeruginosa , biosurfactant, critical micelle concentration (CMC), FT-IR fourier transform infrared spectroscopy (FTIR). African Journal of Biotechnology , Vol 13(31) 3394-3401
Highlights
Re eceived 9 April, 2014; Accepted 4 August, 2014 sent study, biosurfactan b t-producing microorgan isms Pseud domonas aerruginosa PBS. In this pres isolated fro om mangrove ecosystem m in Pichava aram (Boat h house), Tam mil Nadu, India
Stability studies werre done at va arious pH, te emperature and a
The mangrove soil samples were collected from various places of Cuddalore district of Tamil Nadu, India
Summary
Re eceived 9 April, 2014; Accepted 4 August, 2014 sent study, biosurfactan b t-producing microorgan isms Pseud domonas aerruginosa PBS. In this pres isolated fro om mangrove ecosystem m in Pichava aram (Boat h house), Tam mil Nadu, India. M) with crude oil as th production was done using a min nimal salt medium m The microbial growths g were e investigate ed, and the best b cultivati on times forr the biosurffactant produ uction by. P. aerugino osa PBSC1 was w found to o be 144 h. The biosurfa actant was e extracted an nd characterrized. Stability studies werre done at va arious pH, te emperature and a
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
