Abstract
Platycodon grandiflorus is an important medicinal plant in China. Induction of polyploidy was successfully attempted in this medicinal plant using 0.05% colchicine semi-solid under 72 h by apical shoot tip treatment of young seedlings, and then 50% mutant plants were obtained. It was testified by the chromosome number in mitosis and its karyotype analysis for diploid control and obtained mutants. The results showed that the chromosome number of the diploid plant was 2n=2x=18, whereas the chromosome number of the obtained mutants was 2n=4x=36. The karyotype formula of mutant plant and diploid control were K=2n=4X=36=24m+12sm (4SAT) and K=2n=2X=18=12m+6sm (2SAT), respectively by karyotype analysis and they belonged to 2B and 2A karyotype. It was confirmed that the obtained mutant plants were tetraploid. Key words: Platycodon grandiflorus, Obtained mutants, karyotype analysis.
Highlights
Platycodon grandiflorus, a species of perennial flowering plant of the family Campanulaceae, is native to East Asia and has been used as an important medicine or food for centuries in China
Karyotypic characters, mitotic metaphase chromosomes of diploid control and the mutants are as shown in Tables 1 and 2 and Figures 1 to 4, respectively
Analysis of somatic metaphases showed that the chromosome numbers of mutants were 2n=4x=36, while the chromosome numbers of diploid control were 2n=2x=18
Summary
Platycodon grandiflorus, a species of perennial flowering plant of the family Campanulaceae, is native to East Asia (such as China, Korea, Japan, and East Siberia) and has been used as an important medicine or food for centuries in China. According to some recent reports, saponins from the roots of P. grandiflorus (CKS), as one of the most essential functional components in radix, have been shown to exhibit many other pharmacological activities, including anti-cancer (Park et al, 2005; Lee et al, 2004, 2013; Jeong et al, 2010; Chun et al, 2013), antioxidant (Jeong et al, 2010; Ryu et al, 2012), the inhibition of pancreatic lipase (Zhao and Kim, 2004), anti-allergic (Han et al, 2009), anti-hyperglycemiccholesterol (Ahn et al., 2012; Jang et al, 2010), anti-skin photoaging (Hwang et al, 2011), stimulate osteoblast differentiation (Jeong et al, 2010), lowering Cholesterol (Zhao, 2006), and antiobesity effects (Zhao et al, 2006; Hwang et al, 2013).
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