Abstract
Dental caries is a common disease in human population which has a multifactorial etiology. The present study aimed to investigate the antimicrobial efficacy of various extracts of Olea europaea against six bacterial pathogens [Staphylococcus aureus, Streptococcus mutans, Staphylococcus sanguinis, Staphylococcus sobrinus, Staphylococcus salivarius, Lactobacillus acidophilus and one fungi (Candida albicans)]. Plant material was crushed and extracted in petroleum ether, acetone, methanol and aqueous through successive method by using Soxhlet apparatus. The antimicrobial activity of extracts was examined by agar well diffusion method at 200 mg/ml sample concentration and minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) by two fold serial dilution method. Ofloxacin was used as positive control to determine the sensitivity of the strains. The results show that methanol extract was more active than other extracts in its antimicrobial activity. The zone of inhibition exhibited by methanol extract against tested microorganisms ranged between 13.6±0.28 to 20.6±0.28 mm, respectively. Phytoconstituents analysis of plant extract revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, terpenoids and saponins. The results validate the traditional uses of O. europaea in treatment of dental diseases. Key words: Antimicrobial activity, agar well diffusion method, dental pathogens, Olea europaea.
Highlights
L extract e ts against de ental pa athoge ens Sanjjay Kumarr1*, Navnee et1, Murli Manohar
Dental carie study aimed to investig gate the antimicrobial effficacy of va arious extrac cts of Olea e europaea ag gainst six bacterial pathogens p ccus aureus s, Streptoco occus muta ans, Staphy ylococcus sanguinis, Staphyloco occus sobrinus, Staphylo ococcus saliv varius, Lacto obacillus aciidophilus an nd one fungi (Candida albicans)]
The antimicrobial activity off extracts was examined by aga ar well diffu usion metho od at 200 m mg/ml samp ple concentrration and minimum inhibitory concentratio c ns (MICs) and minimum m bactericida al concentra ations (MBCs) by two fo old serial dilution me ethod
Summary
L extract e ts against de ental pa athoge ens Sanjjay Kumarr1*, Navnee et1, Murli Manohar. The objective of present study was to investigate the antimicrobial properties of O. europaea to cure dental diseases. Plant extracts were prepared by immersing separately 200 g of dried powder in 600 ml of four different solvents that is petroleum ether (PET), acetone (ACE), methanol (MeOH) and aqueous (H2O) The antimicrobial activity of different extracts was determined by agar well diffusion method (Perez et al, 1990).
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