Abstract
This study was performed on two races of purslane; agronomic purslane and wild one. All the explants were cultured on MS basal medium supplement with 30 g/l sucrose, 8 g/l agar and different plant growth regulator. Petioles, shoot tips and leaves of wild purslane and also leaves of agronomic purslane were cultured in different concentrations and combinations of IBA and BAP (Both in the same four levels, 0, 1, 5 and 10 µM; collectively 16 treatments). On the other hand, nodal segments and shoot tips of agronomic purslane were cultured in different concentrations (4.44, 8.88 and 13.32 µM) of BAP or kinetin. The regenerated were rooted in the media containing three levels of IBA or IBA (0.0, 2.5 and 5 µM). Results showed that the treatments containing10 µM IBA in combination with 10 or 5 µM BAP are suitable for callus induction from leaves of wild purslane. Direct shoot regeneration from shoot tips or petiole explants of wild purslane was observed only in 10 µM IBA alone. Also, BAP at level 8.88 µM was found to be the best treatment to shoot regeneration from nodal segments of agronomic purslane and IBA at level 2.5 µM was found to be the best treatment for rooting of regenerated shoots in both races of purslane. Key words: Portulaca oleracea, microproagation, callus induction, BAP, NAA, IBA.
Highlights
Purslane (Portulaca oleracea L.) is a pharmaceutical plant that grows in warm and moist regions of north hemispher
Results showed that the treatments containing10 μM indole-3 butyric acid (IBA) in combination with 10 or 5 μM BAP are suitable for callus induction from leaves of wild purslane
MS basal media supplemented with 30 g/l sucrose, 8 g/l agar and different plant growth regulator was prepared in 500 ml conical bottles. pH was adjusted to 5.8 and were autoclaved for 20 min at 121°C
Summary
Purslane (Portulaca oleracea L.) is a pharmaceutical plant that grows in warm and moist regions of north hemispher. Purslane has wide range of pharmaceutical importance. For example it is used as anti bacterial and antivirus matters in China (Okwuasaba et al, 1986). It has wound healing and muscle relaxant activities in mouse muscles Extensive studies have been performed about purslane, such as determination of it effective materials (Liu et al, 2000), but information about tissue culture of this
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
