Abstract
In this paper, adopting orthogonal design method, the Morchella esculenta mycelia from Qinghai-Tibetan plateau was preliminary studied in different growing conditions such as pH, temperature, carbon sources, nitrogen sources and growth factors. The results showed that the M. esculenta mycelium liquid fermentation pH was between 6.0 to 7.0, cultivating optimum temperature 24ËšC, training required for 1.2% of potassium nitrate nitrogen (KNO3), carbon source was 4.5% soluble starch, growth factor biotin was 0.1%. At the same time, we also took the plateau M. esculenta polysaccharide fermentation liquid extraction, the extraction time was optimal combination 2 h, precipitated with ethanol concentration of 90% extraction ratio 1:20, the highest yield of polysaccharides. Key words: Qinghai-Tibetan Plateau, Morchella esculenta, sporocarp, mycelium, static culture.
Highlights
Polysaccharide is the material basis of life, it has a comparable information function of nucleic acid or protein, involved in cell-cell recognition, regulation of immune function and intercellular substance during transport, cell transformation, apoptosis, and most non-toxic
The results showed that the M. esculenta mycelium liquid fermentation pH was between 6.0 to 7.0, cultivating optimum temperature 24 ̊C, training required for 1.2% of potassium nitrate nitrogen (KNO3), carbon source was 4.5% soluble starch, growth factor biotin was 0.1%
Static culture conditions optimized of M. esculenta mycelium
Summary
Polysaccharide is the material basis of life, it has a comparable information function of nucleic acid or protein, involved in cell-cell recognition, regulation of immune function and intercellular substance during transport, cell transformation, apoptosis, and most non-toxic. The research found that polysaccharides had anti-tumor and immunomodulatory (Zhang et al, 2007), anti-bacterial, anti-viral (Li et al, 2003), antioxidant and anti-aging, and many other pharmacological activities, it had become the ideal drug and natural sources of antioxidants. Fungal polysaccharides have been widely used into immunodeficiency diseases, autoimmune diseases, cancer and other diseases and clinical treatment and medical field for other purposes such as preparation of pharmaceutical materials, drug delivery agents, blood plasma substitutes. The study of fungal polysaccharide and its compound has attracted more and more attention. It has become one of the hot research areas such as molecular biology, medicine, food science, etc
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