Abstract
Various factors such as browning, season, media type and plant growth regulators influence the micropropagation of female trees of Myrica esculenta . These factors have successfully been addressed after addition of some media additives, collection of the explant at right season, standardizing the media type and use of plant growth regulators at varying concentrations. Polyvinylpyrollidone (PVP - 0.5%) was found effective for successful partial removal of phenolic compounds and obtaining maximum percent survival of explants. The explant collection season played an important role in reducing phenol induced browning and winter season was found best for explant establishment. The maximum number of shoots (4-5/explant) was obtained in Woody Plant Medium (WPM) supplemented with 10µM kinetin and 0.1µM naphthalene acetic acid (NAA). Of all the media types tried, Woody Plant Medium was found to be the best. Kinetin was found superior to benzyl amino purine and N 6 (γ,γ-dimethylallyamino)purine (2iP) for explant establishment and multiplication. NAA induced rooting to 45.8% of explants in 1/2 strength Woody Plant Medium. However, ex vitro survival percentage was low. Key Words: Browning, in vitro, plant regeneration, tissue culture, woody plant medium. African Journal of Biotechnology Vo l.3(10) 2004: 534-540
Highlights
The popularity of the species can be judged from the fact that local people of the region can earn over Rs. 14 .00 lakh /season from selling the fruits of the species (Bhatt et al, 2000a)
To determine the most suitable hormonal combination for explant establishment and multiple shoot production, sterilized explants were inoculated into test tube each containing 15 ml Woody Plant Medium (WPM) supplemented with various concentration of kinetin (1.0-10 μM) and naphthalene acetic acid (NAA) (0.01-0.1 μM)
To standardize most suitable media for explant establishment and multiple shoot induction, sterilized explants were inoculated into different media type: WPM, MS (Murashige and 1962 medium) (Skoog) 1962), (Gamborgs medium (B5) (Gamborg et al, 1968) and 1/2 MS, containing kinetin (10 μM) and NAA (0.1μM)
Summary
The present study was carried out to investigate the influence of medium additives on explant establishment, season of explant collection, effect of auxin-cytokinin addition on shoot proliferation and effect of auxin on rooting of shoots of mature female trees of M. esculenta. Pretreated explants were cultured in 25 x 150 mm test tubes containing 15 ml WPM supplemented with 10 μM kinetin and 0.1 μM NAA. To determine the most suitable hormonal combination for explant establishment and multiple shoot production, sterilized explants were inoculated into test tube each containing 15 ml WPM supplemented with various concentration of kinetin (1.0-10 μM) and NAA (0.01-0.1 μM).
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