Abstract
The present investigation involved testing the effect of different carbon sources (glucose, sucrose and starch) as well as different nitrogen sources (peptone, yeast extract and urea) on the production of vitamin B12 using Klebsiella pneumonia grown on mixture of agriculture wastes (wheat straw and rice bran). The results show that glucose was the best carbon source compared to the other di- and polysaccharides used. The best vitamin B12 output (63.87 µg/L) was obtained at 3 g/L glucose. On the other hand, peptone appeared to be the most suitable nitrogen, since it gave (57.8 µg/L) of B12 compared to the other nitrogen sources used. Statistical analysis for the obtained results had been carried out and the data showed that glucose and peptone additions were highly significant compared to the other sources under investigation. Key words: Vitamin B12 production, solid state fermentation (SDF), bacteria.
Highlights
Vitamin B12 like other B vitamins is important for the metabolism
The present investigation involved testing the effect of different carbon sources as well as different nitrogen sources on the production of vitamin B12 using Klebsiella pneumonia grown on mixture of agriculture wastes
The results show that glucose was the best carbon source compared to the other di- and polysaccharides used
Summary
Vitamin B12 like other B vitamins is important for the metabolism. It helps in the formation of red blood cells and in the maintenance of the central nervous system (Wickramasinghe, 1995). The deficiency of vitamin B12 is associated with hematologic, neurologic and psychiatric manifestations. It is a common cause of megaloblastic anemia (Atta et al, 2008). Folic acid is necessary for fertility in both men and women. In men it contributes to spermatogenesis but in women enhance oocycte maturation placentation (Ebisch et al, 2007).
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