Abstract
In this study, the microbiological quality and lipid oxidation of the grass carp (Ctenopharyngodon idella) fillets treated by dipping in sodium acetate (0, 1 and 3%), nisin (0, 0.1 and 0.2%) or their combination were evaluated during 16 days of refrigeration storage. Antilisterial effect of nisin was enhanced with the increased concentration of sodium acetate. With increasing the concentrations of sodium acetate, mesophilic counts were lower but regarding nisin, better results were obtained by applying 0.1% nisin. Greater inhibition of mesophile bacteria was observed when combination treatment was used. The number of lactobacillus was lower when higher concentrations of sodium acetate and nisin were used. Peroxide, TBA and total viable base nitrogen (TVB-N) values were lower in the samples treated with both nisin and sodium acetate and higher results were obtained in the combination treatments. Key words: Listeria monocytogenes, nisin, sodium acetate, microbial quality, chemical quality, grass carp.
Highlights
Midkine (MK) is a heparin-binding growth factor found as the product of a retinoic acid-responsive gene (Kadomatsu et al, 1988)
It has 45% sequence identity to pleiotrophin [(PTN)/heparin-binding growth-associated molecule (HB-GAM)], and together, these molecules comprise a family of heparin-binding growth factors (Tomomura et al, 1990)
In this enzyme-linked immunosorbent assay (ELISA), dose-dependent increase in the absorbance at 450 nm enables the measuring of MK ranging between 0.2 and 10 ng/ml
Summary
Midkine (MK) is a heparin-binding growth factor found as the product of a retinoic acid-responsive gene (Kadomatsu et al, 1988). We used the rabbit specific antibodies against human MK to establish a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) of MK system and applied it to detect serum MK levels in different types of cancer patients. The standard curve, precision and recovery rate were tested, respectively, and serum MK concentration of 102 cancers patients and 102 normal individuals were detected using this method.
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