Abstract
To our knowledge, no research study has been carried out on the effects of ascorbic acid (ASA), 5-Aminolevulinic acid (ALA) and Nano selenium (N-Se) on the cytological parameters of pea seedlings under salinity stress. Salinity treatment (60 and 120 mM NaCl) was applied. Two concentrations of ASA (50 and 100 ppm), ALA (25 and 50 ppm), and N-Se (10 and 20 ppm), respectively were used individually and in combination with NaCl (60 and 120 mM). Modifications in shoot length, number of leaves, leaf area, chromosomal aberrations and mitotic index were determined. Salinity treatment (120 mM) caused the highest reduction in shoot length, leaf area and mitotic index. A significant increase of chromosomal abnormalities percentage (%) was detected in salinity treatments compared with control. ASA (100 ppm), ALA (50 ppm) and N-Se (10 ppm) treatments significantly reduced the damaging effect of salinity stress on growth attributes, mitotic index and chromosomal abnormalities percentage (%) and improved seedlings’ performance. These treatments can be recommended for the improvement of pea plants’ productivity under salt stress. Key words: Ascorbic acid, 5-aminolevulinic acid, nano selenium, salt stress, mitosis, chromosomal aberrations, Pissum sativum L.
Highlights
Salt stress adversely affects the morphological, physiological and biochemical responses of plant species (Nazar et al, 2011)
The mitotic index in root tip meristems of P. sativum treated with salt mixture (60 and120 mM) significantly decreased compared to seeds
Cytological analysis showed that, under harmful stress conditions (120mM NaCl), the highest value of mitotic index (%) was observed in pea treated with ascorbic acid at a concentration of 100 ppm (13.53%) followed by Aminolevulinic acid (ALA) 50 ppm (13.19%); nano selenium at a concentration of 20 ppm gave the lowest value (10.12%), compared to control (10.02%)
Summary
Salt stress adversely affects the morphological, physiological and biochemical responses of plant species (Nazar et al, 2011). Ascorbic acid (ASA) is a naturalist product that acts as an antioxidant and enzyme and improves cofactor. It engages in a variety of procedures. ASA has a number of roles in protein modification and cell division in plant cells (Hussein et al, 2019) Nowadays, it plays an essential role in a series of physiological processes such as cofactor of key enzyme, plant defense against oxidization, growth, development, cell division, cell extension, senescence and counteracts the deleterious effects of biotic and abiotic stresses (Zhang and Sonnewald, 2017). It is chosen to be one of the substances of the subject of our present study
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
