Abstract
Bacilli are a large homogeneous group of bacteria that survive in a wide range of environmental conditions. Formation of resistant spores allows them to survive in high temperature zones where other organisms cannot. Eighty samples were collected and inoculated directly into nutrient broth. Of the eighty samples collected, thirty three exhibited growth. Nine of these were Gram positive rods, twenty were Gram negative rods, and four were Gram positive cocci. Only cultures that yielded Gram positive rods were processed further. Antimicrobial profiling was performed using standard organisms: Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, E. coli 35218 and Pseudomonas aeruginosa ATCC 27853. No inhibition was noted against S. aureus ATCC 29213 and E. coli 35218. Five of the nine Gram positive isolates revealed inhibitory properties against the standard organisms. Phylogenetic analysis of amplified 16S rDNA gene confirmed that all the six antagonistic isolates formed close phylogenetic clusters with known members of Bacillus species with a 88-99% sequence identity. The current study shows the presence of thermophilic Bacillus species, which are potential biomolecule producers within the hot springs of Lake Bogoria, Kenya. However, further investigation will be useful for the discovery of novel bioactive substances effective against wide range of pathogens. Key words: Bacillus, Lake Bogoria, secondary metabolites, antibiotics.
Highlights
Rece eived 20 Februarry, 2015; Accepte ed 25 March, 201 mogeneous group of bacteria that survive in a wide range of enviro onmental Bacilli are a large hom o resistant spores s allow ws them to su urvive in high temperature zones wh here other conditions
A gene conffirmed that a all the six a antagonistic c isolates formed clos se phylogen netic clusters s with known members of Bacillus s species with h a 88-99% s sequence identity
Indee ed, applying extremophile es in industrries hass opened a new era in biotechnology
Summary
Rece eived 20 Februarry, 2015; Accepte ed 25 March, 201 mogeneous group of bacteria that survive in a wide range of enviro onmental Bacilli are a large hom o resistant spores s allow ws them to su urvive in high temperature zones wh here other conditions. Of the eighty samples collecte ed, thirty thrree exhibited d growth. A gene conffirmed that a all the six a antagonistic c isolates formed clos se phylogen netic clusters s with known members of Bacillus s species with h a 88-99% s sequence identity. Th he current sttudy shows the presenc ce of thermo ophilic Bacilllus species, which are potential biomolecule producers s within the hot springs of Lake Bo ogoria, Kenya a. Further inve estigation will be usefful for the dis scovery of no ovel bioactiv ve substance es effective a against wide e range of pa athogens.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
