Abstract
Abstract— In this study, the effects of lead (Pb) contamination on artichoke (Cynara scolymus L.) and runner bean (Phaseolus coccineus L.) seedlings determined by physiological, biochemical and molecular parameters. Reduction of root growth, dry weight and total soluble protein content in root of artichoke and runner bean seedlings were observed with the under certain concentrations of lead. However, PCR (Polymerase Chain Reaction) based RAPD fingerprinting techniques was used to investigate the genotoxic effects of metal stress on seedlings for molecular research. Unique polymorphic band patterns of 23 different RAPD primers were used for the RAPD analysis. There were some changes observed in RAPD profiles as variations like increment and/or loss of bands of seedlings which is implemented to heavy metal stress compared to control seedlings. Consequently, genomic template stability had changed according to the RAPD profiles of seedlings under lead (Pb) heavy metal stress.
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