Abstract
A set of 36 polymorphic simple sequence repeat (SSR) primers well distributed on all the 12 rice chromo-somes have been used to assess the genetic diversity among the rice varieties. A total of 98 alleles were detected with an average of 2.78 alleles per locus across 39 genotypes. The number of alleles varied from two to four of these SSR loci. Among the primers, RM 401, RM 20A, RM 536, RM 7575 and RM 5862 produced maximum number of alleles (4). Out of the 98 alleles, 86 were polymorphic and this exhibited 87.76% polymorphism. SSR marker analysis differentiated the genotypes into two distinct groups. The polymorphism information content (PIC) value of the markers ranged between 0.28 and 0.50 with a mean value of 0.45. The highest PIC value (0.50) was observed for ten primers that is, RM 2, RM 24, RM 411, RM 104, RM 266, RM 125, RM 4674, RM 247, RM 3476 and RM 3351 while the lowest was 0.28 for RM 29. The genetic similarity coefficients for 39 genotypes obtained with SSR markers ranged from 23.8 to 78.4%. Key words: Rice, genetic diversity, simple sequence repeat (SSR) markers.
Highlights
A set of 36 polymorphic c simple sequence repea at (SSR) prim mers well disttributed on a all the 12 rice e chromosomes have e been used to assess th he genetic diversity amon ng the rice v varieties
1.0 g of leaf tissue was ground with liquid nitrogen and to this powder 750 μL of preheated (65°C) CTAB buffer was added
Out of 50 SSR markers, 36 markers that were polymorphic in all the genotypes consistently were selected to analyze the variation among the genotypes (Table 2)
Summary
A set of 36 polymorphic c simple sequence repea at (SSR) prim mers well disttributed on a all the 12 rice e chromosomes have e been used to assess th he genetic diversity amon ng the rice v varieties. A to otal of 98 alle eles were detected with an average of 2.78 alleles per locus across 39 genotype es. The e genetic similarity co oefficients fo or 39 genoty ypes obtained d with SSR m markers rang ged from 23.8 to 78.4%. NA based mollecular marke ers are highlyy useful in this ext since theyy are available e in abundancce and clearly y conte allow w the comparisson of geneticc material at juvenile phase e avoid ding any envirronmental influence on gen ne expression n.
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