Abstract
Infection by Erwinia amylovora, the causal agent of fire blight (FB) disease, induces in apple and pear host plants, the generation of reactive oxygen species (ROS). We analyzed at molecular level, the link between ROS production and susceptibility to bacterial infection. Gene expression time course showed that expression of chloroplast, mitochondrial and nuclear genes, whose transcription is redox-dependent, was down regulated or suppressed in tolerant cultivar, Harrow Sweet, in comparison with susceptible cultivar, Williams. Monitoring of oxidative burst by localization of hydrogen peroxide showed that oxidative burst was triggered faster in tolerant cultivar in response to infection. These results suggest that transcription of some redox-dependent genes of cytoplasmic organelles and nucleus, in the two cultivars, is regulated faster in the tolerant cultivar than in the susceptible one. Key words: Chloroplast, electron transport Chain, Erwinia amylovora, mitochondria, Pyrus communis, redox.
Highlights
Erwinia amylovora in both compatible and incompatible interactions secretes three types of effector proteins: HrpN, HrpN/W and disease specific type III effector protein (DspA/E) (Bogdanove et al, 1998; Kim and Beer, 1998; Wei et al, 1992)
There is biochemical evidence indicating the role of chloroplast electron transport chain (ETC) in oxidative burst of plantpathogen interactions (Abdollahi and Ghahremani, 2011), and likewise Samuilov et al (2002) proved that mitochondria and chloroplast are required in programmed cell death (PCD) of guard cells in pea leaves
They showed that illumination stimulated the CN-induced destruction of guard cells that contain both mitochondria and chloroplasts, but not of epidermal cells that contain mitochondria only
Summary
Erwinia amylovora in both compatible and incompatible interactions secretes three types of effector proteins: HrpN, HrpN/W and DspA/E (Bogdanove et al, 1998; Kim and Beer, 1998; Wei et al, 1992). There is biochemical evidence indicating the role of chloroplast ETC in oxidative burst of plantpathogen interactions (Abdollahi and Ghahremani, 2011), and likewise Samuilov et al (2002) proved that mitochondria and chloroplast are required in programmed cell death (PCD) of guard cells in pea leaves. They showed that illumination stimulated the CN-induced destruction of guard cells that contain both mitochondria and chloroplasts, but not of epidermal cells that contain mitochondria only
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