Abstract
This study was designed to elucidate the signalling pathways by which secretary phospholipase a2 (sPLA2s) induce in vitro neutrophil migration and PLA2 inhibits platelet aggregation in PRP and explains the decreased clot retraction and retarded and compromised elasticity build up. Naja naja venoms are complex and contain several toxic components, including neurotoxins and phospholipases A2 that cause post-synaptic neuromuscular blockade with respiratory paralysis and cardiac arrest. The antigenic epitopes on phospholipase a2 (Pla2) are important determinant of protection against venom. In this analysis, we found the antigenic epitopes 29-GRGGSGTPVDD-39, 77-TCKGDNNACA-86, of protein called the antigenic determinant or the epitope is sufficient for eliciting the desired immune response. Also predict the MHC binder and these MHC Class peptide segments are from a set of aligned peptides known to bind to a given major histocompatibility complex (MHC) molecule as the predictor of MHC-peptide binding. Binding ability prediction of antigen peptides to MHC class molecules is important in vaccine development. The method integrates prediction of peptide MHC class binding; proteasomal C terminal cleavage efficiency of protein. Keywords - phospholipases a2, epitope, hydrophobicity, MHC, surface activity, SVM, PSSM, peptide vaccine
Highlights
Venomous animals contain concentrates of biologically active substances developed to block vital physiological and biochemical functions of the victims
Our research studies strongly define the target model, which suggests protein-protein interaction rather than proteinphospholipid interaction determines the pharmacological specificity of Phospholipase A2 (PLA2) enzymes [3, 4]
We found the SVM based MHCII-IAb peptide regions; MHCII-IAd peptide regions; MHCII-IAg7 peptide regions and MHCII- RT1.B peptide regions, which represented predicted binders from phospholipase A2 protein (Table 2)
Summary
Venomous animals contain concentrates of biologically active substances developed to block vital physiological and biochemical functions of the victims This has contrasting human health concerns, provide important pharmacological raw material and pose a serious threat to human life and health in tropical and subtropical regions. Phospholipase A2 (PLA2) is the superfamily consists of many different groups of enzymes that catalyze the hydrolysis of the sn-2 ester bond in a variety of different phospholipids This reaction produces a free fatty acid and lysophospholipid have many different important physiological roles 2. The structure-function relationships and the mechanism of this group of small proteins are subtle, complex and intriguing challenges It is strongly anticoagulant CM-IV binding properties to factor Xa (its target protein) through the specific anticoagulant site on its surface. The method integrates prediction of peptide MHC class binding affinity, hydrophobicity and relative flexibility of given protein molecule [5, 6]
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