Abstract

Nannochloropsis gaditana is a microalgae belonging to the class of Eustigmatophyceae. This particular microalga is the most studied species. For its richness in lipids, it is used for the biodiesel production. The aim of this work is to advance another important metabolite which is cellulose. This represents 25% of the dry weight of the N. gaditana. A low concentration of NaOH (2%) and sodium hypochlorite (6%) allowed the obtainment of relatively pure cellulose analyzed by Fourier transform infrared (FT-IR) and X-Ray Diffraction (XRD). The progress of the microalgae mass production could help in the substitution of the cellulose of microalgae for the vegetal cellulose, as seen in the simple technical extraction, the yield and the procurement of uncontaminated molecule with lignin. This substitution will contribute in protecting the environment. Keywords: Cellulose, Nannochloropsis gaditana, procedure extraction, structural characterization.

Highlights

  • Nannochloropsis gaditana is a microalgae that belongs to the class of Eustigmatophyceae (Andersen, 1998)

  • Cellulose obtained after extraction and dried is 25% of the dry weight of the microalgae, N. gaditana

  • The Fourier transform infrared (FTIR) analysis of the cellulose extract identified the functional groups of the cellulose and its contaminants

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Summary

Introduction

Nannochloropsis gaditana is a microalgae that belongs to the class of Eustigmatophyceae (Andersen, 1998). It has five species and they are: N. gaditana, N. salina, N. oculata, N. oceanica and N. limnetica (Hibberd, 1981; Lubian,1982; Karlson et al.,1996; Krienitz et al.,2000 Suda et al.,2002). N. gaditana is rich in lipids and produces mass biodiesel (AttilioConverti et al, 2009; Rodolfi et al, 2009; Pal et al, 2011; Bondioli et al, 2012). The cell wall of N. gaditana is comprised a bilayer structure consisting of a cellulosic inner wall (∼75% of the mass balance), protected by an outer hydrophobic algaenan layer (Scholz et al, 2014). Reasons being that: (i) the growth of microalgae is quick and harvesting is done continually throughout the year (Gouveia, 2011); (ii) they can grow in saline aqueous areas, waste water and brackish water (Gouveia, 2011)

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