Abstract
Ageratum conyzoides Linn is a medicinal plant used for diverse ethnomedicinal applications including anti-ulcer treatment. Usually, protection of gastric mucosa from injury or ulceration is dependent on the efficacy of intrinsic or induced protective factors against erosive effects of aggressive factors. In this study, our aim was to ascertain the gastroprotective activity of methanolic leaf extracts of A. conyzoides L. and assess the associated roles of gastric mucous cells and p53 protein. This study involved 25 adult male Wistar rats divided into five groups (A-E). Groups A and E were used as normal and test controls while B-D were administered with extracts at 100, 300 and 500 mg/kg body weight for 28 days. Gastric mucosal injury was induced via pyloric ligation method. Gastric tissues were processed, stained with periodic acid-Schiff and immunostained for p53 protein (using monoclonal antibody). Stained sections were quantified using image-J software, data obtained were statistically analyzed. The results showed significant increase (p<0.05) in mucous cell population but no significant increase in p53 protein expression in gastric tissues of treated animals. This implied that increase in mucous cell count and down-regulation of p53 protein in gastric tissues play key role in gastroprotective activity of methanolic extracts of A. conyzoides L. Key words: Ageratum conyzoides, mucous cell, p53 protein, gastroprotection, rats.
Highlights
Ageratum conyzoides Linn (Asteraceae) is a medicinal, tropical plant commonly found in West Africa and parts of Asia and South America
This study was carried out to ascertain gastroprotective activity of methanolic leaf extracts of A. conyzoides L. and to determine the role of gastric mucous cells and tumor suppressor (p53) protein during the activity in male Wistar rats treated with the extracts prior to induction of gastric mucosal injury through pyloric-ligation method
The Periodic acid–Schiff (PAS)-stained tissue sections showed the distribution of mucous cell population in the gastric mucosa of experimental animals in normal control group A, treated groups B-D and test control group E (Figures 1 and 2)
Summary
Ageratum conyzoides Linn (Asteraceae) is a medicinal, tropical plant commonly found in West Africa and parts of Asia and South America It is an annual herbaceous plant not usually eaten by humans largely because of the characteristics bad odour, likened to the smell of a male goat but has an age-long record of diverse traditional medicinal uses in several countries (Ming, 1999; Okunade, 2002; Shekhar and Anju, 2012). It is popularly known for its usage in treatment of burns, wounds, infectious diseases, arthritis and fever (Kamboj and Saluja, 2008). This study was carried out to ascertain gastroprotective activity of methanolic leaf extracts of A. conyzoides L. and to determine the role of gastric mucous cells and tumor suppressor (p53) protein during the activity in male Wistar rats treated with the extracts prior to induction of gastric mucosal injury through pyloric-ligation method
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