Abstract
Potato soft rot is one of the major destructive diseases affecting potato plants throughout the world. In a survey of different potato growing seasons in different regions of Jordan, samples of rotten potato tubers were collected and 131 isolates identified biochemically as Pectobacterium carotovorum subsp. carotovorum (Pcc). The PCR primer pair (EXPCCR/EXPCCF) was used to detect these Jordanian isolates. The primer set amplified a single fragment of 550 bp in size from the total genomic DNA, which was extracted independently from 67 Pcc strains. In a nested PCR, the primer set (INPCCR/INPCCF) amplified the expected single fragment of 400 bp from the PCR product of first PCR amplification. The use of these primers was not reliable in detecting all isolates identified biochemically as Pcc. Different rots causal agents were detected by PCR amplification and further sequenced. The sequencing data revealed similarities to different genera; Pseudomonas, Enterobacteriaceae genera such as Enterobacter spp., Serratia spp. and Klebsiella spp., in addition to P. carotovorum subsp. carotovorum. So far this is the first study where Pcc has been identified by using PCR and sequencing approaches in Jordan. Key words: Pectobacterium carotovorum subsp. carotovorum, specific primers, nested PCR, sequencing.  
Highlights
Different bacterial diseases have been reported to attack potatoes around the world leading to high economic losses in yield and quality under favorable environmental conditions; of these are brown rot (Ralstonia solanacearum), common scab (Streptomyces scabies), ring rot (Clavibacter michiganensis subsp. sepodonicus), black leg (Pectobacterium carotovorum subsp.atrosepticum) and soft rot (Pectobacterium carotovorum subsp. carotovorum).potato soft rot is one of the most important diseases of potatoes; it causes a great reduction in yield resulting in economic losses in field and during transit
The sequencing data revealed similarities to different genera; Pseudomonas, Enterobacteriaceae genera such as Enterobacter spp., Serratia spp. and Klebsiella spp., in addition to P. carotovorum subsp. carotovorum
The bacterial isolates isolated from rotted potato from different regions were all identical in their cultural and biochemical properties and similar to that of reference culture of Pectobacterium carotovorum subsp. carotovorum (Pcc) isolate NCPPB312
Summary
Potato soft rot is one of the most important diseases of potatoes; it causes a great reduction in yield resulting in economic losses in field and during transit. Soft rot disease causes greater total losses of produce than any other bacterial disease, it is considered among the factors contributing to yield losses, and among the most prevalent and destructive bacterial diseases that affect vegetables which is difficult to control (Perombelon and Kelman, 1980; Bhat et al, 2010b)
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