English

Cabrera Jova Manuel,Medero Vega Victor,De Feria Manuel,Goacute Mez Kosky Rafael,Rayas Cabrera Ayme,Loacute Pez Torres Jorge,Basail Perez Milagros

doi:10.5897/ajb09.1252

Abstract

English

Highlights

In vitro propagation of yam by serial culture of single node cuttings has been used in the rapid multiplication of diseases-free material in elite seed yam programs (Malaurie et al, 1995; Borges et al, 2004, Ondo et al, 2007)
Plants from microtubers formed in temporary immersion systems (TIS) and in vitro plants of yam cloneBelepdid not show variability in morphological characters in comparison to plants obtained from conventional propagation methods as control
Yam plants from microtubers developed in a temporary immersion system showed good performance under field conditions, without significant differences in comparison with in vitro plants with respect to tuber number, tuber length, tuber diameter and tuber fresh weight per evaluated plant 36 weeks after culture, but differences were noticed with plants from tuber crowns

Summary

Introduction

In vitro propagation of yam by serial culture of single node cuttings has been used in the rapid multiplication of diseases-free material in elite seed yam programs (Malaurie et al, 1995; Borges et al, 2004, Ondo et al, 2007). The protocols of yam by serial culture of sail-node cuttings have presented low multiplication coefficients and survival of the plants in the acclimatization phase and in field (Malaurie et al, 1995; Medero et al, 1999; Chu and Ribeiro, 2002; Borges et al, 2004). The alternative end-product in the yam micro propagation process is a small tuber (microtuber) produced when in vitro plantlets are placed under tuber-inducing conditions (Mantell and Hugo, 1989; Salazar and Beltran 2003; Balogun, 2009). They do not need a time consuming hardening period in a greenhouse and may be adapted to largescale mechanised planting in the field (Coleman et al, 2001; Pruski et al, 2003)

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