Abstract
Spirulina is useful to man in many aspects of life including health, food and cosmetics. In the present study, we aimed at investigating the optimum physical and chemical conditions that promote Spirulina mass production by varying a set of physical and chemical parameters, namely pH levels; Mg2+ ion concentration; nitrogen, phosphorous and carbon sources; salinity and different growing media. Temperature, light intensity, and light/dark cycle were maintained at 30°C±2, 4400 lx and 14:10 respectively throughout the study. At pH 9, the dry weight, protein, and chlorophyll a contents were 7.83±0.29 g/L, 1.34±0.12 mg/mL and 18.64±0.06 µg/mL respectively. At 0.8 mmol/L MgO, maximum dry weight, protein, and chlorophyll a contents were obtained. By using NaNO3 as the source of nitrogen, the dry weight was 2.24±0.13 g/L while the protein and chlorophyll a contents were 3.24±0.30 mg/mL and 2.53±0.24 µg/mL respectively. The highest biomass, chlorophyll a and protein contents were obtained by using K2HPO4 as the source of phosphorous. When NaHCO3 was used as the carbon source, the highest dry weight, protein and chlorophyll a yields were observed. Of the growing media used, Zarrouk’s medium yielded the highest biomass protein and chlorophyll a contents. Furthermore, under different salinities, the optimal dry weight, protein and chlorophyll a yields were obtained at 2.5%. This study provides the basis for high biomass production of Spirulina which is a promising microalga endowed with many health benefits due to its high protein content. Key words: Biomass, chlorophyll a, protein, Spirulina.
Highlights
IntroductionSpirulina platensis is a planktonic photosynthetic filamentous cyanobacterium that forms massive populations in tropical and subtropical water bodies that have high level of carbonate, bicarbonate and pH values Constituents
Spirulina platensis is a planktonic photosynthetic filamentous cyanobacterium that forms massive populations in tropical and subtropical water bodies that have high level of carbonate, bicarbonate and pH values ConstituentsComposition (g/L)NaHCO3 NaNO3 K2HPO4 K2SO4 NaClCaCl2.2H2O Na2EDTA MgSO4.7H2O FeSO4.7H2O A5 micronutrient sol.a0.04 0.08 0.2 0.01 1 mL/L aA5 micronutrient solution consists of H3BO3, 2.86; MnCl2.4H2O, 1.81; ZnSO4.7H2O, 0.222; CuSO4.5H2O, 0.079; (NH4)6Mo7O24 (g/L)
Effect of different pH levels on biomass production of Spirulina pH is one of the main factors influencing the abundance of inorganic carbon, dissolved total carbon (DTC)
Summary
Spirulina platensis is a planktonic photosynthetic filamentous cyanobacterium that forms massive populations in tropical and subtropical water bodies that have high level of carbonate, bicarbonate and pH values Constituents. NaHCO3 NaNO3 K2HPO4 K2SO4 NaCl. CaCl2.2H2O Na2EDTA MgSO4.7H2O FeSO4.7H2O A5 micronutrient sol.a. 0.04 0.08 0.2 0.01 1 mL/L aA5 micronutrient solution consists of H3BO3, 2.86; MnCl2.4H2O, 1.81; ZnSO4.7H2O, 0.222; CuSO4.5H2O, 0.079; (NH4)6Mo7O24 (g/L). NaNO3 Na2β-glycerophosphate.5H2O Fe-EDTA solution Trace metal solution Thiamine (Vit. B1) Biotin (Vit. H) Cyanocobalomin (Vit. B12). Primary stock solution (see recipe below) (see recipe below) 500 mg/L dH2O 50 mg/L dH2O 25 mg/L dH2O. Quantity (1 L) 3.85 g 0.4 g 100 mL 20 mL 8.0 mL 8.0 mL 3.5 mL. Molar concentration in final medium 4.53×10-4 mol/L 1.31×10-5 mol/L
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
