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Abstract
Reef HQ Aquarium (Townsville, Australia) pumps its new exhibit seawater from a tidal inlet. This study presents the in-house calibration of a bioassay based on juvenile mysids to do a rapid assessment (presence or absence) of toxicity in the new seawater. Calibration tests were carried out for several substances: copper, sodium lauryl sulphate (SLS or SDS), ammonia, antifouling paint, bilge oil from a yacht, oil from a dive compressor, seawater cooling effluent from a commercial ferry vessel, and aquarium tank water. Results were compared with two other bioassays already in use at Reef HQ Aquarium, based on (a) artemia hatched from aquaculture cysts and (b) Vibrio fischeri bacteria (Microtox®). This study determined (a) that the juvenile mysids bioassay yielded meaningful results and was viable operationally, (b) its sensitivity with respect to likely local pollutants, and (c) how it compares in terms of sensitivity with the artemia and the Microtox® bioassays. Key words: ARTOX, bioassay, mysid, Microtox®, Reef HQ Aquarium, seawater, toxicity.
Highlights
Reef HQ Aquarium (Townsville, Australia), the National Education Centre for the Great Barrier Reef, hosts the largest living coral reef tank in the world (2.5 ML), called the Coral Reef Exhibit (CRE aquarium)
Wherever enough data points were available for the mysids and artemia bioassays, a linear extrapolation was done on Figure 4 to identify 4 levels of mortality as follows:
This is the level defined as maximum acceptable for mysids and artemia bioassays at Reef HQ Aquarium. - The Lethal Concentration 50 (LC50) defined as the level of substance that results in a standardised mortality of 50% after 24 h exposure. - The Lethal Concentration 90 (LC90) defined as the level of substance that results in a standardised mortality of 90% after 24 hours exposure
Summary
Reef HQ Aquarium (Townsville, Australia), the National Education Centre for the Great Barrier Reef, hosts the largest living coral reef tank in the world (2.5 ML), called the Coral Reef Exhibit (CRE aquarium). Since 2002, the aquarium has been pumping water from Ross Creek, a tidal inlet on its doorstep, as a supply of new seawater. Water is pumped from the wharf alongside the aquarium building, about 1 km upstream from the creek mouth. This “creek water intake” takes place every two weeks when water is pumped continuously on either side of the high spring tide for 6 to 7 h
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