Abstract
Fruit coloring is a major source of anthocyanin and carotenoid in mango. The anthocyanidin synthase (ANS) is a key enzyme in the anthocyanin biosynthesis. The ANS gene was cloned from mango peel by homologous cloning method. This manuscript reported characterization of ANS from mango peel that comprised 1,262 bp full-length cDNA with open reading frame (ORF) of 1,056 bp and encoding a protein of 351 amino acids. The theoretical molecular weight of the deduced amino sequence of ANS was 39.8 kDa. It was found that the gene encoding for the protein had close relationship with mountain grape (Vitis vinifera), cocoa (Theobroma cacao), mulberry (Morus alba), litchi (Litchi chinensis), and other fruit trees through phylogenetic analysis. Expression of ANS was maximum in the green rather than the yellow and red fruit peel. Expression was down-regulated in mature fruit, there was no response to fruit coloration which was affected by anthocyanin. Key words: Cloning, expression, anthocyanidin synthase (ANS), mango.
Highlights
The anthocyanins are one of the most widespread studied members of the tricyclic flavonoid family of secondary metabolites in plants (Grotewold, 2006)
In a series of studies on recombinant anthocyanidin synthase (ANS) from Arabidopsis thaliana evidence was presented for the initial oxidation of the substrate at C-3 (Welford et al, 2001)
Reverse transcription was achieved with oligo-d (T)18 primers by using 3 μg of total RNA from peel of mango litchi and the primeScript II 1st strand cDNA
Summary
The anthocyanins are one of the most widespread studied members of the tricyclic flavonoid family of secondary metabolites in plants (Grotewold, 2006). The structural genes that encode enzymes involved in the anthocyanin pathway and many regulatory genes for transcriptional regulation of the structural genes have been cloned and characterized from a wide variety of plants (Mol et al., 1998; Holton and Cornish, 1995; Lesnick and Chandler, 1998). Anthocyanidin synthase (ANS), an enzyme of the biosynthetic pathway to anthocyanin, catalyzes the reaction(s) from the colorless leucoanthocyanidins to the colored anthocyanidins. In a series of studies on recombinant ANS from Arabidopsis thaliana evidence was presented for the initial oxidation of the substrate at C-3 (Welford et al, 2001)
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