Abstract
The diversity of culturable bacterial and fungal communities was assessed from leaves, trunk and rhizosphere of coconut palms infected and non-infected by the Cote d’Ivoire lethal yellowing (CILY) phytoplasma. Bacterial and fungal microbes were isolated from leaves, trunk and rhizosphere samples collected from two villages of Grand-Lahou, Braffedon and Yaokro, by using a high-throughput 'dilution-to-extinction' cultivation method coupled with PCR and sequencing with primers that amplified both the 16S ribosomal RNA and intergenic transcribed spacer (ITS) genes. The relative abundance was higher for Bacillus and Candida in Braffedon, and Burkholderia and Neodeightonia in Yaokro. Commonly genera identified from rhizosphere included Bacillus, Burkholderia, Pseudomonas, Streptomyces, Cryptococcus, Penicillium, Purpureocillium and Trichoderma. The most abundant endophytes identified were Pantoea, Candida, Cryptococcus, Bacillus, Pseudomonas, Penicillium, Aspergillus and Rhodotorula. Genera limited to symptomless palms included Arthrininum, Chaetomium, Phialemonium, Fusarium, Klebsiella and Candida. Results indicate that the CILY phytoplasma may be a factor determining the level of diversity of a microbial community in a given location. Our research provides the basis to investigate the possible effect of endophytic and rhizosphere microbes against the CILY phytoplasma to further effectively improve the management of CILY in Grand-Lahou. Key words: Cote d’Ivoire lethal yellowing phytoplasma, endophytes, biocontrol, coconut.
Highlights
Grown in tropical and subtropical regions, the versatile coconut (Cocos nucifera L.) is an important source of Morales-Lizcano et al.food, shelter, and household income for smallholder farmers around the globe (Batugal et al, 2005)
The present work aimed at characterizing the culturable bacterial and fungal communities residing in leaves, trunk, and the rhizosphere of Côte d‟Ivoire lethal yellowing disease (CILY) phytoplasma-infected and CILY phytoplasma-free coconut palms by using the 'dilution-to-extinction' cultivation method coupled with PCR and sequencing approaches
The six coconut palms exhibiting stage 1 (S1), stage 2 (S2) and stage 3 (S3) CILY symptoms collected from Yaokro tested positive for the presence of the CILY phytoplasma with the specific primers G813/AwkaSR
Summary
Grown in tropical and subtropical regions, the versatile coconut (Cocos nucifera L.) is an important source of Morales-Lizcano et al. Endophytes refer to endosymbionts that colonise the inside of the plants without causing any disease to the plant host (Schulz and Boyle, 2006; Wilson, 1995) They can inhabit different plant parts such as tubers, stems, leaves and roots, and can enter the plant via insect sucking or by passive diffusion or active selection from the adjacent rhizosphere (Romanazzi et al, 2009). A member of the genus Cordyceps has been reported as a biocontrol against Lecopholis coneophora, the causal agent of coconut root grub (Kumar and Aparna, 2014) Endophytic bacteria such as Pseudomonas, Bacillus, and Enterobacter have been commonly isolated from a wide range of monocots and dicots including coconut (Rajendran et al, 2008). The present work aimed at characterizing the culturable bacterial and fungal communities residing in leaves, trunk, and the rhizosphere of CILY phytoplasma-infected and CILY phytoplasma-free coconut palms by using the 'dilution-to-extinction' cultivation method coupled with PCR and sequencing approaches
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
