Abstract
In this study, the extraction conditions of polysaccharides of Artemisia mongolia (PAM) were optimized and their molecular weight, weight distribution, composition and antioxidant activities of the polysaccharides investigated. The degree of polymerization of fructan-oligosaccharides 2-8 was separated by thin layer chromatography (TLC) with good results. Polysaccharides were extracted from the leaves of A. mongolia and the effects of the extraction parameters (extraction temperature, extraction time and water to raw material ratio) were optimized under optimal extraction combinations. Based on the study, the maximum yield of polysaccharides (17.38%) was obtained at an extraction temperature of 76.90°C, extraction time of 1.33 h and water-to-raw material ratio of 4.00 ml/mg. The yield of polysaccharides was largely dependent on the extraction parameters. Four isolated fractions of the polysaccharides were further characterized using high-performance liquid chromatography. Using TLC analysis, the degree of polymerization of fructan-oligosaccharides 2-8 was successfully separated. High A. mongolia antioxidant polysaccharide with antioxidant activity of 351.3 sc/g was obtained at optimized incubation temperature of 70.37°C, extraction time of 1.45 h and water-to-raw material ratio of 2 ml/mg. Key words: Artemisia Mongolia, crude polysaccharide, antioxidant activity, response surface methodology, box-Behnken design  
Highlights
Artemisia plant, known locally in Chinese Mandarin Language as “mengguhao” and scientifically as Artemisia mongolica, was used in this study
The leaves of A. mongolica were collected in March 2011 from Songnen grassland in Jilin Province, China, and identified by Professor Yifei Yang of Northeast Normal University, Changchun, China
The model was applicable in the optimization of the processes of A. mongolica polysaccharide extraction
Summary
Known locally in Chinese Mandarin Language as “mengguhao” and scientifically as Artemisia mongolica, was used in this study. A. mongolica leaf is a traditional medicinal herb in China with an early history dating back to the “Nei Meng Gu Zhong Cao Yao” era. The genus Artemisia belongs to the family composite.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
