Abstract
One of the factors that make the treatment of Enterococcus faecalis infections difficult is their ability to form biofilm, as well as their natural and acquired resistance to antibiotics which does not have specific drugs for their inhibition. This fact makes essential the search for alternative treatments, as the use of probiotics strains of Lactobacillus rhamnosus has been effective in the treatment of some diseases. In this investigation, the relationship between the probiotic strain of L. rhamnosus and E. faecalis during the biofilm formation was analyzed. Standardized suspensions used in biofilm development and treatment in different stages of the biofilm formation were prepared. The L. rhamnosus suspension was placed in contact for 90 min with E. faecalis freshly created biofilms (initial adherence) in the 24 h biofilms. The same was made with E. faecalis suspension on L. rhamnosus biofilms. L. rhamnosus showed no inhibitory effects on E. faecalis biofilms formation, with an increase in the counting of colony forming units in the treated groups (p=0.0047, p=0.0060). About the L. rhamnosus biofilms, there was no significant difference for both treatment stages. The probiotic strain interfered in vitro with the E. faecalis biofilm formation, thereby intensifying the growth of E. faecalis biofilm. Key words: Enterococcus faecalis, Lactobacillus rhamnosus, biofilm, virulence factor, probiotic.
Highlights
Enterococcus faecalis is the main cause agents of nosocomial infections and even being present on human intestinal microbiota, has been related to many cases of infections in imunossupressed individuals and or/those treated by broad-spectrum antibiotics
There was no significant interference of E. faecalis on biofilm formation by L. rhamnosus (90 min, p=0.5751 and 24 h, p=0.2300) (Figure 2)
The present study is considered to evaluate if the L. rhamnosus probiotic strain would be capable to interfere with the growth of E. faecalis biofilms hindering its formation or reducing the number of cells, as well as if E. faecalis could interfere with the biofilm formation by L. rhamnosus
Summary
Enterococcus faecalis is the main cause agents of nosocomial infections and even being present on human intestinal microbiota, has been related to many cases of infections in imunossupressed individuals and or/those treated by broad-spectrum antibiotics. The relationship between the probiotic strain of L. rhamnosus and E. faecalis during the biofilm formation was analyzed. L. rhamnosus showed no inhibitory effects on E. faecalis biofilms formation, with an increase in the counting of colony forming units in the treated groups (p=0.0047, p=0.0060).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
