Abstract
Seventy-five species and 3 species varieties belonging to 21 fungal genera were collected from air, soil and leaf surface of broad bean plant on dicholran chloramphenicol malt extract agar (DCMA) and dichloran Rosebengal chloramphenicol agar (DRBC) at 28°C. The results obtained from leaf surface (phyllosphere and phylloplane), soil and atmosphere were basically similar in the two types of media and the most common fungi were: Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium cladosporioides, Cladosporium sphaerospermum and Drechslera neergaardii. The monthly counts of these fungi on two types of media irregularly fluctuated giving maxima value at various months. A. flavus was the highest fungi that produced both exo- and endo-ß-1,4-glucanases among the 9 tested isolates. Maximum production of two enzymes by A. flavus was 8 and 6 days after incubation at 30°C with culture medium containing glucose and cellulose as carbon sources and sodium nitrate as nitrogen source and initially adjusted to pH 6. Key words: Airborne fungi, soil and leaf surface, broad bean, cellulolytic ability.
Highlights
Food legumes play an important and diverse role in the farming systems and in the diets of poor people around the world
During the growing season of broad bean crop which extended from December 2011 to April 2012, a broad bean field in South Valley University in Qena city in Upper Egypt was selected to study the mycoflora of leaf surface and soil, as well as the airborne fungi over the broad bean field
Thirty-four species and 3 species varieties belonging to 15 genera were collected from phyllosphere (10 genera and species + 2 varieties) and phylloplane (12 genera and species +1 var.) of broad bean leaves on dicholran chloramphenicol malt extract agar (DCMA) and dichloran Rosebengal chloramphenicol agar (DRBC) at 28°C (Table 1)
Summary
Soil and leaf surface fungi of broad bean and cellulolytic ability in Upper Egypt. Seventy-five species and 3 species varieties belonging to 21 fungal genera were collected from air, soil and leaf surface of broad bean plant on dicholran chloramphenicol malt extract agar (DCMA) and dichloran Rosebengal chloramphenicol agar (DRBC) at 28°C. The results obtained from leaf surface (phyllosphere and phylloplane), soil and atmosphere were basically similar in the two types of media and the most common fungi were: Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium cladosporioides, Cladosporium sphaerospermum and Drechslera neergaardii. The monthly counts of these fungi on two types of media irregularly fluctuated giving maxima value at various months. A. flavus was the highest fungi that produced both exo- and endo-ß-1,4-glucanases among the 9 tested isolates.
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