Abstract
Cupressus sempervirens L. (Cupressaceae) leaves, cones and young branches have been used in traditional medicine and aromatherapy. The composition of the isolates obtained by hydrodistillation from the aerial parts (plant material collected at Bainam forest, in Northwest of Alger, Algiers), were analyzed by GC and GC-MS. The leaves isolate (yield 0.22% w/w), was mainly composed of monoterpene hydrocarbons (60.8%), α-pinene (38.4%), δ-3-Carene (13.9%), α-Cedrol (10.6%), α-Terpinyl acetate (3.5%) and E-Totarol (3.0%). The cones isolate (yield 0.34% w/w) was predominantly composed of monoterpene hydrocarbons (33.I8%), with α-pinene (20.3%), δ-3-Carene (6.0%), Tepinene-4-ol (9.0%), α-Terpineol (9.0%), α-Terpinyl acetate (5.9%), α-Cedrol (9.1%), and E-Totarol (4.4%). The major components of stems isolates (yield 0.03% w/w) are rich in diterpenoids (51.9%), namely: α-pinene (5.9%), α-Cedrol (14.4%), Manool (5.6%), E-Totarol (34.7%), Ferrugenol (6.0%). Isolates were also tested against four bacteria (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli), and two yeasts (Saccharomyces cerevisiae and Candida albicans), using the Kirby Bauer disk-diffusion method. All bacteria were susceptible to the C. sempervirens volatiles isolates. Antioxidant activity of the isolate was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method together with two antioxidant standards, butylated hydroxytoluene (BHA) and tert-butyl-4-hydroxy toluene (BHT). The results show antioxidant effect of all isolates less significant as BHA and BHT. Key words: Cupressus sempervirens, GC/SM, chemical composition, Antimicrobial activity, Antioxidant activity, using 1, 1-diphenyl-2-picrylhydrazyl (DPPH).
Highlights
Identtificatio on of volatile v e comp pound ds, antiimicrobial p properrties an nd antioxidant actiivity from lea aves, c cones and stems s of o Cup pressus s semp pervire ens fro om Alg geria
The co omposition of the isolattes obtained d by hydrodistillation from the ae erial parts (p plant materia al collected at Bainam fforest, in No orthwest of Alger, Algie ers), were analyzed by b gas chromatography (GC) and GC-MS
The aim of this work was to study the composition of essential oils from tree organs: leaves, stems and cones, of horizontalys C. sempervirens that grow in Algeria and the measure of the antimicrobial and antioxidant activities
Summary
Identtificatio on of volatile v e comp pound ds, antiimicrobial p properrties an nd antioxidant actiivity from lea aves, c cones and stems s of o Cup pressus s semp pervire ens fro om Alg geria. The co omposition of the isolattes obtained d by hydrodistillation from the ae erial parts (p plant materia al collected at Bainam fforest, in No orthwest of Alger, Algie ers), were analyzed by b GC and GC-MS. Th he leaves isolate i (yielld 0.22% w w/w), was m mainly comp posed of monoterpen ne hydrocarbons S s, GC/SM, che emical compo osition, Antim microbial activiity, Antioxidan nt activity, Key words:: Cupressus sempervirens using 1, 1-diiphenyl-2-picrrylhydrazyl
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