Abstract
Alstroemeria with beautiful and long shelf life of cut flower is one of the most important ornamental plants. This plant could propagate by splitting of the in vivo produced rhizomes but the propagation rate is rather low. In the present study, regeneration ability of plantlets was compared using in vitro and in vivo grown rhizome buds as explants. In vitro and in vivo grown rhizome buds were cultured on MS basal medium with 3 different compositions of growth regulators (1, 0.2 mg l-1 NAA with 1 mg l-1 BA and 0.2 mg l-1 IAA with 1 mg l-1 BA). Cultures were incubated in 18 ± 1°C at 16 h photoperiod. Four subculture of explants were done on the same fresh media with 3 weeks intervals. The results showed that in vivo rhizome bud produced the largest number of small rhizome and roots on medium containing 0.2 mg l-1 NAA with 1 mg l-1 BA. Key words: Alestroemeria, in vitro rhizogenesis, shoot proliferation.
Highlights
Alstroemeria is a rhizomatous monocot belonging to the family Alstroemeriaceae (Chiari and Bridgen, 2000)
Segments from the vegetative stem and rhizome buds of Alstroemeria were tested as initial explants
Rhizome buds of Alstroemeria gave the best response as initial explants in in vitro conditions (Lin and Monette, 1987)
Summary
Alstroemeria is a rhizomatous monocot belonging to the family Alstroemeriaceae (Chiari and Bridgen, 2000). Shoot proliferation, number of produced rhizome and root from each explant were evaluated. Our finding showed (Table 1) that rhizome buds explants obtained from pot plants (in vivo) could results in higher number of shoot proliferation (3.97) compared with those derived in vitro (1.83).
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