Year-end Sale % OFF 
Abstract
The research made comparative studies on five media for bacterial isolation. This study aimed at capturing important comparative data in the various types of media for growth efficiency and specific bacteria identification in clinical microbiology. The sources of the samples were urine, nasal swabs and stool. The totality of 15 samples was plated monthly and 120 samples were studied during an eight month period. The mean bacterial load from the cultures grown over the period from each source was calculated and used for comparative growth efficacy. Dominant colonies were characterized and identified based on morphological features and biochemical tests. A 0.1 ml of 10-3 of each bacteria isolate was evaluated for growth potential in triplicate on three different special purpose media. The mean bacteria load from the triplicate cultures was calculated. Salmonella-Shigella agar (SSA); a selective medium had the highest number of bacterial colonies of 2.98 x 105 CFU/ ml followed by the enrichment medium; blood agar that had 2.96 x 105 CFU/ ml and MacConkey agar (MCA) with 2.93 x 105 CFU/ ml. Biochemical identification and characterization of four dominant isolates confirmed the presence of Staphylococcus aureus, Escherichia coli, Salmonella and Shigella. Growth potential of each medium on the bacterial isolates showed that MacConkey agar recorded the highest growth potential of 8.9 x 105 CFU/ ml for E. coli followed by Blood Agar that gave 8.8 x 105 CFU/ml for Shigella. The third highest growth potential of 8.6 x 105 CFU/ ml was recorded in nutrient agar against S. aureus. Statistically, there exists a significant difference among the mean of the five media in their support for bacteria growth at α =0.05. Key words: Bacterial isolation, isolates, Escherichia coli, Shigella, Blood agar.
Highlights
Compa arative studie es on five f culture m media ffor bac cterial iso olation n
The mean bacterial load from urine, stool and nasal swab samples cultured on different plating media showed that
Sa almonella-Shigella agar (SSA); a selective medium had the highest number of bacterial colonies of 2.98 x 105 CFU/ ml followed by the Enrichment medium that had 2.96 x 105
Summary
Compa arative studie es on five f culture m media ffor bac cterial iso olation n. This study aimed at capturing im mportant co omparative data in the va arious types s of media fo or growth effficiency and d specific bacteria ide entification in n clinical microbiology. The. T sources s of the samp ples were urrine, nasal sw wabs and stool. The totality of 15 samples was w plated monthly m and d 120 sample es were studied during an eight month perio od. The mea an bacterial load from th he cultures g grown over tthe period frrom each source was calculated and used for f compara ative growth h efficacy. 10-3 of each h bacteria isolate was s evaluated for f growth potential p in triplicate on n three differrent special purpose me edia. Sa almonella-Shigella agar (SSA); a selective medium m had the highest number of bacterial b collonies of 2.9
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
