Abstract

Green synthesis of silver nanoparticles (AgNPs) is non-toxic and eco-friendly than commonly used physicochemical methods. The study focuses on synthesis, characterization, antibacterial and antioxidant activity of AgNPs synthesized using aqueous extract of Moringa stenopetala (M. stenopetala) leaves. Optimum heating time required for the preparation of aqueous extract of M. stenopetala leaves was 15 min. It was found that 90 ml of 1 mM of silver nitrate solution and 15 ml of aqueous extract of M. stenopetala leaves were more favorable for maximum production of AgNPs with a yield of 97.14%. Ultraviolet-visible (UV-Vis) spectrum of synthesized AgNPs shows a peak at 412 nm. Fourier transform infrared spectroscopy (FTIR) spectral analysis indicated involvement of -C=O, -O-H, and -N-H in the formation of AgNPs. X-ray diffraction (XRD) pattern showed the face centered cubic (FCC) structure of AgNPs with average particle size of 11.44 nm. Synthesized AgNPs showed stronger antibacterial activity against Escherichia coli than Staphylococcus aureus and has better antioxidant activity than standard ascorbic acid. Key words: Silver nanoparticles (AgNPs), green synthesis, Moringa stenopetala.

Highlights

  • Nanotechnology is a broad field of science that represents the design, synthesis, characterization and application of materials at nanoscale level which can be used across various fields such as chemistry, biology, physics, material science, medicine, etc. (Surya et al, 2016; Jassim et al, 2016) and nanoparticles (NPs) are viewed as fundamental building blocks of nanotechnology (Vastrad and Goudar, 2016)

  • When the heating time of extract was more than 15 min, there was a decrease in maximum absorbance of AgNPs; higher heating time degraded the phytochemicals present in the M. stenopetala leaves

  • The present study indicates that the optimal heating time required for the preparation of aqueous extract of M. stenopetala leaves was 15 min

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Summary

Introduction

Nanotechnology is a broad field of science that represents the design, synthesis, characterization and application of materials at nanoscale level which can be used across various fields such as chemistry, biology, physics, material science, medicine, etc. (Surya et al, 2016; Jassim et al, 2016) and nanoparticles (NPs) are viewed as fundamental building blocks of nanotechnology (Vastrad and Goudar, 2016). Nanotechnology is a broad field of science that represents the design, synthesis, characterization and application of materials at nanoscale level which can be used across various fields such as chemistry, biology, physics, material science, medicine, etc. (Surya et al, 2016; Jassim et al, 2016) and nanoparticles (NPs) are viewed as fundamental building blocks of nanotechnology (Vastrad and Goudar, 2016). The term “nanoscale” is generally referred to a scale between 1 and 100 nm (Lakshman et al, 2016). Green synthesis of silver nanoparticles (AgNPs) provides advancement over physicochemical methods as it is cost effective, eco-friendly, scaled up and there is no need to use high energy, temperature and toxic chemicals (Sadeenp et al, 2016).

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