Abstract
Phenotypic and phylogenetic studies were performed for the facultative alkalophile from the rhizosphere of Ipomoea pes caprae, a plant growing on coastal sand dunes. The isolate was Gram positive and showed optimum growth at pH 10.5. Chemotaxonomic analysis revealed that the isolate contained type B1 peptidoglycans with L-lysine as the diamino acid; rhamnose and galactose were the cell wall sugars and belonged unambiguously to the genus Microbacterium. The major menaquinones were MK-11 and MK-12. The 16S rDNA sequence of the Microbacterium arborescens isolate was deposited in the GenBank with an accession number DQ287961. The phylogenetic and phenotypic distinctiveness of the strain indicates it as a novel Microbacterium sp., named as M. arborescens AGSB. Key words: facultative alkaliphile, Microbacterium arborescens AGSB, Coastal sand dune vegetation, Ipomoea pes caprae, 16S rRNA sequencing.
Highlights
The sand dune ecosystem is a stressed habitat with only certain type of vegetation surviving in this ecosystem, one such plant is Ipomoea pes caprae which is commonly found on coastal dunes
Chemotaxonomic analysis revealed that the isolate contained type B1 peptidoglycans with L-lysine as the diamino acid; rhamnose and galactose were the cell wall sugars and belonged unambiguously to the genus Microbacterium
The phenotypic characteristics of the strain are in agreement with the placement of the strain in the genus Microbacterium
Summary
The sand dune ecosystem is a stressed habitat with only certain type of vegetation surviving in this ecosystem, one such plant is Ipomoea pes caprae which is commonly found on coastal dunes. The isolates obtained in this study have been identified as Acinetobacter, Pseudomonas, Paenibacillus, Microbacterium, Agrobacterium Chryseobacterium and Pseudomonas (Park et al, 2005, 2006; Leveau et al, 2009; Godinho and Bhosle, 2010; Muthezhilan et al, 2012; Gaonkar et al, 2012). Earlier studies on isolates from this ecosystem have shown their ability to produce exopolymers which aid in sand aggregation and stabilize the dunes (Godinho and Bhosle, 2009). Predominant facultative alkaliphile from the rhizosphere of Ipomoea pes caprae that exhibited orange, pigmented colonies and gram positive, non sporing regular rods which has previously not been reported. A new species is proposed as Microbacterium arborescens AGSB
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