Abstract
The conditions for immobilizing the new alkaline protease-producing bacteria strainTeredinobacter turnirae by entrapment in calcium alginate gel were investigated. The influence of alginate concentration (20, 25 and 30 g/l) and initial cell loading (ICL) on enzyme production were studied. The production of alkaline protease improved significantly with increasing alginate concentration and reached a maximum enzyme yield of 8000 U/ml at 25 g/l alginate concentration. This was about 176.8% higher than that obtained by free cells (2890 U/ml). The immobilized cells produced alkaline protease consistently over 5 repeated cycles and reached a maximal value of 9000 U/ml on the third cycle. This was 311.4% (3.11-fold) as compared with the control (free cells). Simple mass balance analysis was applied to describe the growth and the protease production behaviour of both fractions the cells in free form and the entrapped in Ca-alginate beads. Scanning electron microscope studies indicated the internal distribution pattern of the cells encapsulated in Ca-alginate beads. The results presented in this paper show the potential for using immobilized T. turnirae cells in Ca-alginate for the production of a novel alkaline protease. Key words: Alkaline protease, Ca-alginate, immobilization, Teredinobacter turnirae, repeated batch.
Highlights
Alkaline proteases have found a wide application in several industrial processes such as an additive to detergents and in bating of hides and skins in leather industries (Godfrey and Reichet, 1985)
In order to find out the optimum alginate concentration for T. turnirae cell immobilization, alginate solutions of different concentrations (20, 25 and 30 g/l) were used
The production of alkaline protease improved with increasing alginate concentration and reached a maximum yield of 8000 U/ml at 25 g/l alginate (Figure 1A)
Summary
Alkaline proteases have found a wide application in several industrial processes such as an additive to detergents and in bating of hides and skins in leather industries (Godfrey and Reichet, 1985). They hold more than 50% of the total enzyme market (Sachidanandham et al, 1999). The protease produced has been isolated from a symbiotic bacterium found in the gland of Deshayes of the marine shipworm (Griffin et al, 1992). It retains a high level of activity above 50°C.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
