Abstract
Corynebacterium sp was isolated from the soil by using 3-5 dinitro benzoic acid (DNB) as a sole carbon source. The highest rate of degradation of aniline (AN) or DNB was found in the exponential phase of the growth of the bacterium. After 24 h, about 50% of DNB and 30% of AN were degraded by Corynebacterium sp. At a concentration of 0.5 to 1 g/L of AN or DNB, good growth was obtained and the protocatechuic acid was detected. The optimum concentration of yeast extract was 2 g/l. Catechol 1-2 dioxygenase was induced in the cells grown on a medium containing AN or DNB. A significant activity of this enzyme was detected, which means that ortho cleavage pathway may be present inCorynebacterium sp. Key words: Corynebacterium sp., degradation, dinitrobenzoic acid (DNB), aniline (AN),protocatechuic acid, catechol, catechol 1-2 dioxygenase, Ortho cleavage, high performance liquid chromatography (HPLC).
Highlights
Majority of organic compound residues cause environmental hazards
Corynebacterium sp was isolated from the soil by using 3-5 dinitro benzoic acid (DNB) as a sole carbon source
Bacterial strains in the degrading culture were purified by repetitive streaking onto minimal salt medium (MSM); 1 g/l DNB was added as sole carbon source and pure colony was obtained after 6 days
Summary
Majority of organic compound residues cause environmental hazards. Nitroaromatics and aniline are toxic chemicals present in the effluent of many industries as they are widely used as raw materials in the manufacturing of a number of products such as dyes, plastics, resins, pharmaceuticals, petro-chemicals, herbicides, pesticides among others. (Nishino and Spain, 2004, 2006; Peres and Agathos, 2000). Many nitroaromatics have been shown to be toxic or mutagenic to many bacteria, yeasts, fungi, unicellular algae, todepool copepods and oyster larvae (Won et al 1976; Marvin-Sikkema and de Bont, 1994). This organism is capableof utilizingdifferentnitroaromaticcompounds.Bengtsson and Carlsson (2001) isolated bacteria from pristine groundwater that degraded aniline in a slow rate. Liu et al (2002) isolated a bacterial strain, AN3, which was able to use aniline or acotanilide as sole carbon and nitrogen sources from activated sludge and identified it as Delftiasp. Corynebacterium sp isolated from the Egyptian soil which is able to grow on dinitrobenzene and aniline and detects the cleavage pathway of these compounds
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