Abstract
Since antimicrobials are used to fight infectious diseases, their proper use is a major world concern. The use of more than one medicine in the treatment of infectious disease is controversial hence the association therapy is used empirically to treat severe infections when one antimicrobial alone is not sufficient to account for all possible pathogens involved in the infection. The importance of understanding the mechanisms of gentamicin and ampicillin antibiotic action, as well as their interactions has important therapeutic implications. This research aimed to verify isolated and simultaneous antimicrobial action “in vitro” of gentamicin and ampicillin on strains of Enterococcus sp. in patients with Enterococcus sp. Fifty strains of Enterococcus sp. were used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) of ampicillin and gentamicin, both isolated and associated, from which the fractional inhibition coefficient (FIC) was determined. A modified checkerboard method was also used for evaluating the association of the two antimicrobial agents. The results revealed that the antibiotics have a time- and dose-dependent response, and ampicillin has better results isolated than when in association. The results indicate a potential therapeutic failure in 32% of the associations in these agents. Thus, the most effective pharmaceutical care is facilitated by determining the antibiotics concentrations. Determining the MIC, MBC and FIC can be a powerful tool for guiding strategies to prevent bacterial resistance through the rational use of antibiotics, thereby averting and avoiding therapeutic failures. Key words: Drug antagonism, drug synergism, Enterococcus sp., microbial sensitivity tests.
Highlights
Pharmaceutical Care is a set of actions aimed at the promotion, protection and recovery of health, both of individuals and society, relying on the use of medicines and drugs as an essential resource and aiming at their access and rational use
In the experiment involving the use of ampicillin alone in cells cultured TSB medium with decreasing concentrations of ampicillin (0.2; 0.1; 0.05; and 0.025 mg/mL), it was demonstrated that the lowest antibiotic concentrations: 0.1; 0.05; and 0.025 mg/mL showed inhibition of bacterial growth in 38, 35 and 34 tubes, respectively
If we extrapolate this result “in vitro” to “in vivo” conditions, it would imply that more diluted concentrations of ampicillin would not be indicated, since they could lead to a risk of bacterial resistance, some susceptibility to ampicillin was observed at a higher concentration
Summary
Pharmaceutical Care is a set of actions aimed at the promotion, protection and recovery of health, both of individuals and society, relying on the use of medicines and drugs as an essential resource and aiming at their access and rational use. As well as their selection, programming and evaluation of their use, with a view to achieving concrete results and improving the population's quality of life (MS, 2004). Pharmacotherapy succeeds when results such as disease prevention, control, cure, normalization of laboratory parameters and/or relief of symptoms are achieved as expected (Correr et al, 2011)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
