Abstract

Environmental alterations leads to fluctuations in the soil microbial population and soil enzyme activities, as different weather parameters affect microbial biota and their activities in rhizospheric soil of crop. An agroclimatic study was carried out to study the effect of environmental alterations on soil microbial population and enzyme activities in rhizospheric soil of rice and wheat crop. Rice and wheat crop were grown under field conditions and under temperature gradient tunnel which was maintained at 4-5°C higher temperature than open field temperature. Soil samples were taken from rhizospheric soil of rice and wheat. Microbial population was enumerated by serial dilution spread plating technique. Statistically significant higher microbial population of total bacteria, nitrogen fixers and P-solubilizers was found in rhizospheric soil samples taken from temperature gradient tunnel as compared to soil samples taken from rice and wheat grown under field conditions. Fungal population was found to be statistically, significantly higher in soil samples taken from field conditions in case of rice but, higher in soil samples taken from temperature gradient tunnel in case of wheat crop. Activities of alkaline phosphatase and dehydrogenase were assessed by using para nitro phenl phosphate (PNPP) and tri phenyl tetrazolium chloride (TTC) as substrates, respectively. Enzymes activities were found to be significantly higher in rhizospheric soil samples taken from temperature gradient tunnel. Microbial population and enzyme activities were found over a broad range of temperature but, maximum microbial and enzyme activity was found only at and near optimum conditions. Key words: Environmental alterations, microbial activities, rice, temperature gradient tunnel, wheat.

Highlights

  • IntroductionAn agroclimatic study was s carried out to study the effect of environmen ntal alteratio ons on soil m microbial population and enzyme e activities in n rhizospherric soil of ric ce and wheatt crop

  • Effects of clim mate change on pla ant ass sociate ed mic crobiall com mmuniities an nd enzzyme a activitie es Jupinder

  • Sta atistically significant higher micro obial population of total bacteria, nittrogen fixers s and P-solu ubilizers was found in rhizospheriic soil samp ples taken fro om temperatture gradien nt tunnel as compared to o soil samples taken from rice and a wheat grown g underr field condiitions

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Summary

Introduction

An agroclimatic study was s carried out to study the effect of environmen ntal alteratio ons on soil m microbial population and enzyme e activities in n rhizospherric soil of ric ce and wheatt crop. Sta atistically significant higher micro obial population of total bacteria, nittrogen fixers s and P-solu ubilizers was found in rhizospheriic soil samp ples taken fro om temperatture gradien nt tunnel as compared to o soil samples taken from rice and a wheat grown g underr field condiitions. Fung gal populatio on was foun nd to be sta atistically, significantly y higher in soil s samples taken from field f conditio ons in case o of rice but, h higher in soill samples taken from temperature e gradient tu unnel in cas se of wheat c crop. Natural and a antthropogenic factors have e dramaticallly altered the t com mposition of the atmosph here, which may m

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