Abstract
Moderate halophilic bacteria from Dowma al Jandal in extreme north Al Jouf were isolated using cultural-dependent methods. Halophiles from hypersaline environments possess a biotechnological potential. Polymerase chain reaction (PCR) amplification of the 16s rRNA gene and phylogentic analysis were used to identify strains. Culturing was done aerobically in chemically defined media (CDM). Salt concentration (15%) was used at pH 7.2. Universal bacterial primers were used to amplify 16SrDNA from chromosomal DNA isolated from the four distinct colonies. Four moderate halophilic bacterial isolates were analyzed and identified wih 16srDNA sequencing as Virgibacillus salarius, Bacillus subtilis, Bacillus sp., and Virgibacillus marismortui. Comparison of the 16srDNA sequence alignment to reference sequence data bases showed samples M1, M2, M3 and M4 have 95-99% homology. All of the four isolates had at least 95% similarity to the published sequences implying that they could be species within the described genera. Key words: Moderate halophiles, hypersaline lake, Bacillus sp., 16srDNA sequencing.
Highlights
B us sp. isolate i ed from m athalosalin ne lake e (Al Jouf, Saudi Arabia a)
A 100 μL aliquot of each diluted sample was spread on chemically defined medium (CDM) agar (15-20% NaCl w/v) plates
The four distinct moderate halophilic bacteria obtained from the saline soil of the Dowma al Jandal Lake designnated as M1, M2, M3 and M4 were analyzed
Summary
B us sp. isolate i ed from m athalosalin ne lake e (Al Jouf, Saudi Arabia a). Jouf were isolated using g culturaldependent methods. Isolate i ed from m athalosalin ne lake e (Al Jouf, Saudi Arabia a) Kushner (1978) defined the moderate halophiles as organisms growing optimally between 0.5 and 2.5 M salt. Recent studies showed that an extensive research work has been done on isolation and characterization of large number of moderately halophilic Gram-positive, endospore-forming aquatic isolates in the genus. PCR amplification and 16s ribosomal RNA sequencing
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