Abstract
The present work aimed to evaluate the antibacterial activity of aqueous and methanolic extracts of Coriaria myrtifolia’s leaves against Agrobacterium sp. and Agrobacterium tumefaciens “plant pathogen responsible for crown gall” in an objective to identify novel antimicrobial agents and to put forward efforts of proving plant’s extracts scientific credibility, and determining their spectrum of activity. The bacteria tested were found profoundly sensitive to both of the C. myrtifolia extracts. The extent of inhibition was more important by methanolic extract than by aqueous one. The average diameter of inhibition zones ranged from 10.67 to 15.33 mm and 12.68 to 18 mm for aqueous and methanolic extract, respectively. This study was the first to report the antimicrobial activity of extracts obtained from the leaf of C. myrtifolia against Agrobacterium sp. and Agrobacterium tumefaciens. It can be concluded that the observed antibacterial characteristics of C. myrtifolia indicate that it might be a promising antimicrobial agent. Key words: Antibacterial, bacteria, plant extracts, Coriaria myrtifolia, Agrobacterium tumefaciens.
Highlights
Pesticides are an essential input for preventing pre and post harvest crop losses (Saksena, 2001)
The largest diameter of inhibition zone was observed for methanolic extracts on the growth of S13PC6
The inhibition activity of the antibiotic (T+) was not significantly different from that of the aqueous extract of Oued el Koub. These results indicate that under the same operating conditions and for the same strain, the antibacterial activity of C. myrtifolia extract from Bab Berred was much greater than that of C. myrtifolia extract from Oued el Koub, as well as for aqueous extract or methanolic extract
Summary
Pesticides are an essential input for preventing pre and post harvest crop losses (Saksena, 2001). Incessant and extensive use of these synthetic pesticides is inducing serious problems to the life supporting systems due to their residual toxicity (Ferrer and Cabral, 1991; Andrea et al, 2000). The large numbers of synthetic pesticides have been banned in the developed countries because of their undesirable attributes such as high and acute toxicity, long degradation periods, accumulation in the food chain and extension of their power to destroy both useful and harmful pests (Barnard et al, 1997; Ortelli et al, 2005). In-spite of using all available means of plant protection, about 1/3 of the yearly harvest of the world is destroyed by pests and the induced loss is expected to be nearly $300 billion per year (Chandler, 2005). Many phytopathogenic bacteria have acquired resistance to synthetic pesticides (Williams and Heymann, 1998; White et al, 2002)
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