Abstract
Leptin gene has its role in appetite, metabolism, growth and milk production in cattle. Single nucleotide polymorphisms (SNPs) in leptin gene in different cattle breeds have been reported and subsequently associated with their production performance. The objective of this study was to evaluate the association of genetic differences in the bovine leptin gene with milk production, reproduction, milk constituents in Sahiwal and Frieswal cattle of India. In total, one hundred and seventy six cows were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to screen the presence of three SNPs in leptin gene. The testing of Hardy- Weinberg equilibrium for the three SNPs of within Frieswal and Sahiwal population indicated that the polymorphism site in the populations fitted with Hardy-Weinberg equilibrium (P > 0.05) except for C/BspEI/T and C/NruI/T position in Sahiwal. Polymorphism C/NruI/T have significant association with age at first service and age at first calving and heterozygotes have more prolonged age at fist service and age at first calving. For milk protein, C/BspEI/T and C/HphI/T was found to have significant effect. For lactose and SNF, C/HphI/T polymorphism has found to be significant. In case of combined genotyping, genotype CTCTCC (713.00±167.99 days) was found to have noticeable higher age at first service and age at first calving. But milk production higher first lactation yield was noted for CCCCCT (3987.00±337.86 kg). Key words: Leptin gene, polymorphism, Frieswal, Sahiwal, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), combined genotype.
Highlights
Remarkable progress has been achieved in milk production since 1980, due to the intense selection of animals based on the production performance
The objective of this study was to evaluate the association of genetic differences in the bovine leptin gene with milk production, reproduction, milk constituents in Sahiwal and Frieswal cattle of India
One hundred and seventy six cows were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to screen the presence of three single nucleotide polymorphisms (SNP) in leptin gene
Summary
Remarkable progress has been achieved in milk production since 1980, due to the intense selection of animals based on the production performance. This resulted in the declining trend in various non-yield traits like reproductive performance which in turn resulted in the low economic output of the dairy farmers. Identification of single nucleotide polymorphisms (SNP) opened new vistas in animal breeding as these methods are quite cheaper and resulted in direct genotyping for candidate genes using polymerase chain reaction (PCR) (Karen et al, 1998; Mataves et al, 2003). Leptin is a 167 amino acid or 16 kDa polypeptide, Abbreviations: WBC, White blood cells; PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; SNP, single nucleotide polymorphisms. Buchanan et al 2002
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