Abstract
The performance of a hybrid of Saccharomyces cerevisiae and Saccharomyces paradoxus (Yeast B) compared with an industrial strain of S. cerevisiae (Yeast A) was studied. The two strains of yeasts: Yeast A obtained from the Bioresources Development Centre, National Biotechnology Development Agency Ogbomosho and Yeast B obtained from Scotch Whisky Research Laboratory in Edinburgh, Scotland (Strain 63M) were studied using the SLSF method for bioethanol production at various initial starch concentrations (20, 30 and 50%). The cassava starch used was extracted from Tropical Manihot esculenta (TME 419) cassava strain while the SLSF method for bioethanol production was initiated by adding granular starch hydrolysing enzyme with the yeast strains into the starch solutions at room temperature. The results obtained showed that Yeast B has higher bioethanol yield and tolerance generating 70.34, 87.34 and 120.53 ml/L compared to the Yeast A which produced 63.4, 72.73 and 112.6 ml/L at 20, 30 and 50% starch concentrations respectively. The study suggests that the hybrid strain of S. cerevisiae and S. paradoxus out performs the industrial strain and can favorably substitute or displace the industrial strains for bioethanol production. Key words: Bioethanol, cassava, fermentation, hybrid, simultaneous liquefaction saccharification and fermentation (SLSF), yeast, Saccharomyces cerevisiae, Saccharomyces paradoxus.
Highlights
Biofuel has been known to be an excellent replacement for fossil fuels
Compositions of the fermentation media used for the bioethanol production were soluble glucose, yeast extract, ammonium sulphate (NH4(SO4)2), potassium di-hydrogen phosphate (KH2PO4), magnesium sulphate (MgSO4.7H2O), copper sulphate (CuSO4. 5H2O), zinc sulphate (ZnSO4), iron sulphate FeSO4, manganese sulphate (MnSO4), with starch concentrations ranging from 1 - 10% used when testing for the effect of different substrate concentrations
The yeast strains used for fermentation were a new hybrid strain of S. cerevisae and S. paradoxus (Strain 63M) collected from Scotch Whisky Research Laboratory at Edinburgh, Scotland and an industrial strain of S. cerevisae collected from the National Biotechnology Development Agency (NABDA)’s Bioresources Development Centre (BIODEC) at Ogbomoso, Oyo State which is being used at the bioethanol plant at the Centre
Summary
Biofuel has been known to be an excellent replacement for fossil fuels. This is because the latter are by nature non-renewable and have numerous environmental hazards associated with its extraction and utilization (Solomon et al, 2018). Nigeria with a population approximated to be 200 million (National Bureau of Statistics, 2018) has been faced with challenges of sufficiently meeting its ever-increasing energy demands. With an average daily fuel consumption of about 51 million liters in 2016 to about 54 million litres in the first quarter of 2018 (Petroleum Products Consumption Statistics, National Bureau of Statistics, Qtr 2, 2018), there is absolute need for an alternative fuel to supplement and curtail our over reliance on fossil based energy sources as a Nation. Bioethanol is the most popular alternative fuel that has found applications both as transportation fuel and in industries
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