English

P Somerita,A A,K B P,N Chatterjee S,Yopadhyay Yopadhyay

doi:10.5897/ajb12.2703

Abstract

  In this study, a total number of fifteen moribund Labeo bata were collected from Hooghly, Purba Medinipur, and South 24 Parganas districts of West Bengal, India. The lesions and hemorrhages were recorded at the base of the fins or on the skin due to bacterial infection. Isolation and pure culture of the causative agent were made in the laboratory. The 16S rRNA gene sequence of the bacterial isolate PB112 has been deposited in the NCBI GenBank and was assigned the accession number JN996498. The GC and AT contents of the 16S rRNA gene sequence (JN996498) were 54.22 and 45.78%, respectively. Based on the biochemical properties, 16S rRNA gene sequence analysis and fatty acid methyl ester (FAME) analysis, the causative bacteria were identified as Pseudomonas aeruginosa.   Key words: Labeo bata, Pseudomonas sp., 16S rRNA gene sequence, fatty acid methyl ester (FAME) analysis.

Highlights

Bata (Labeo bata Ham.) is one of the most favorite and popular fish among minor carps having high market value
Bacterial disease outbreaks impose a significant constraint in fish and shellfish production (Bachere et al, 1995)
The fatty acid composition of the bacterial isolate PB112 was compared to a Sherlock Library of known organisms in order to find the closest match

Summary

Introduction

Bata (Labeo bata Ham.) is one of the most favorite and popular fish among minor carps having high market value. Several species of Pseudomonas have been reported to cause disease in a number of fish species, including goldfish like Carassius auratus (Bullock, 1965), and are associated with septicemia in aquatic animals (Roberts, 1978). A number of aquatic animals like fish, frogs and soft-shelled turtles were recorded to be susceptible to Pseudomonas spp. The present study aimed at determining the phenotypic, biochemical and molecular properties of the disease-causing bacterial isolate.

Results

Conclusion