Abstract
Callus cultures were induced from leaf and stem explants of Artemisia absinthium, at different auxin and cytokinin concentrations. Moderate concentrations of growth regulators either in combination or in single in MS medium produced friable, light green and non-embryogenic callus from both explants. These totipotent cells gave rise to shoots when transferred to same or different growth regulator containing medium as second subculture. Complete rooting was achieved on full and half strength basal MS medium supplemented with different auxin concentrations. Synergetic effect of plant growth regulator plays an important role in callus induction and cell differentiation.
Highlights
Medicinal plants are source of important therapeutic aid for alleviating human ailments (Dev, 1997)
Artemisia absinthium L. commonly known as wormwood or “vilayati afsanteen” is a perennial herb growing in the northern hilly areas of Pakistan (Haq, 1983)
Micropropagation of A. absinthium has been previously established by using shoot tips (Nin et al, 1996), as an alternative, the culture of callus tissue provides an important tool that can be preliminary step in the regeneration of whole plants
Summary
Plant Physiology Laboratory, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan 45320. Callus cultures were induced from leaf and stem explants of Artemisia absinthium, at different auxin and cytokinin concentrations. Moderate concentrations of growth regulators either in combination or in single in MS medium produced friable, light green and non-embryogenic callus from both explants. These totipotent cells gave rise to shoots when transferred to same or different growth regulator containing medium as second subculture. Complete rooting was achieved on full and half strength basal MS medium supplemented with different auxin concentrations. Synergetic effect of plant growth regulator plays an important role in callus induction and cell differentiation
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