English

Retana-Márquez Socorro,Romero Carlos,Chemineau Philippe,López Gabriela,Juárez-Rojas Lizbeth,Keller Matthieu,Solano Floriberta,Alberto Delgadillo José,Hernández Eunice,Casillas Fahiel

doi:10.5897/ajpp2016.4682

Abstract

The administration of Mesquite pod extract (containing mesquitol, daidzein and genistein) to female and male rats disrupts reproductive variables. However, its effect on fertility is not known. This study evaluated fertility in male and female rats treated with mesquite pod extract, comparing its effects with those of daidzein and estradiol. The following treatments were given for 30 days to groups of female and male rats: vehicle, mesquite pod extract, DAI and E2. Treatments were administered subcutaneously for 30 days. These extract disrupted both the female and male sexual behavior in a similar way to DAI, but less than E2. Mesquite pod extract increased the number of days in estrus and decreased lordosis intensity during proestrus. Mesquite pod extract-treated males showed lower testicular and glandular weights, as well as decreased sperm motility, viability and count. In females treated with mesquite pod extract, the number of pups was lower than in control females, and 10 to 20% of pups were dead. These effects were similar to those with DAI-treatment. Despite the lower sperm quality, the fertility of mesquite pod extract- and DAI-treated males seem not to be disrupted, as they could impregnate control females. These results show that mesquite pod extract can disrupt female but not male fertility. Key words: Mesquite pod extract, daidzein, fertility, offspring, sexual behavior, phytoestrogens.

Highlights

Discovery of clover disease in ewes (Bennetts et al, 1946) consumption of feed containing large amounts of plant oestrogens has been suspected to cause temporary or permanent fertility problems in ruminants
A recent study evaluated the effects on conception and early gestation of nulliparous ewes fed for five months before gestation with red clover rich in the phytoestrogen formononetin
The current study shows that mesquite pod extract adversely affects pregnancy outcome, the extract did not impair pregnancy

Summary

Introduction

Discovery of clover disease in ewes (Bennetts et al, 1946) consumption of feed containing large amounts of plant oestrogens has been suspected to cause temporary or permanent fertility problems in ruminants. Has been reported that low intake of food with high content of isoflavones causes temporary infertility, and prolonged consumption can cause permanent infertility (Marshall, 1973; Adams, 1990; Adams, 1995). A recent study evaluated the effects on conception and early gestation of nulliparous ewes fed for five months before gestation with red clover rich in the phytoestrogen formononetin. Heifers fed clover had a lesser conception rate, and a greater percentage of heifers returning to estrus than silage-fed heifers. This indicates that isoflavone content in clover disturbs hormonal balance during early pregnancy, leading to a reduction in the fertility of heifers (Hashem et al, 2016)

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