Abstract
A novel bacterium pigmented isolate from Caatinga soil was characterized by biochemical and molecular assays, as well as, by rep-polymerase chain reaction (PCR) and 16S rDNA sequencing, and was identified as Serratia marcescens based on 99% of similarity. The identity of the sequences were compared by pairs of critical species of S. marcescens found in National Center for Biotechnology Information (NCBI) with a 96% homology of the isolated species to species database. The wild strain was able to produce biosurfactant (BS) using cassava (Manihot esculenta Crantz) wastewater, with addition of lactose, and corn oil, according to full factorial design 23, at 28°C, and static condition. The net liquid metabolic reduced the surface tension of the water from 70 to 30.60 mN/m during the stationary phase (assay eight constituted by 6.0% cassava wastewater, 1.0% lactose and 7.5% corn oil), and produced emulsifier agent (4.012 UEA) at the same condition. This study identified the pigmented bacterium as new strain of S. marcescens, and showed it has potential to promote both emulsions formation and surface tension reduction in cassava wastewater (6.0%), lactose (1.0%) and corn oil (7.5%) proved as an alternative economical medium for commercial biosurfactant processes. Key words: Serratia marcescens, tensio-active, bioemulsifier, cassava wastewater.
Highlights
The semi-arid region of Northeast of Brazil comprises approximately 969.589.4 of km2 (Sousa et al, 2015)
The results identified the strain as Enterobacteriacea family, genus Serratia (Table 2)
The biosurfactant produced by S. marcescens in the best condition of factorial design evaluated by surface tension of cell-free metabolic net showed stability under all different pH, NaCl concentrations and temperature
Summary
The semi-arid region of Northeast of Brazil comprises approximately 969.589.4 of km (Sousa et al, 2015). Res. soil composition those under native vegetation for semi-arid is latosols and luvisols under native vegetation for representative sequences from Pernambuco (Sá et al, 2015). Soil composition those under native vegetation for semi-arid is latosols and luvisols under native vegetation for representative sequences from Pernambuco (Sá et al, 2015) This region has a dry tropical deciduous vegetation, constituted by small trees, bushes and grasses, xerophiles, where deciduous plants are the mainly vegetation. It is characterized by high level of insolation, high temperature, scanty hydric resources and scarce rains, which often cause long periods of drought. All these factors are usually unfavorable for microbial growth in the soil; bacteria constitute the principal group of decomposers responsible for carbon recycling (Solanki and Arora, 2015)
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